RGD Reference Report - Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis. - Rat Genome Database

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Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis.

Authors: Motulsky, E  Koch, P  Janssens, S  Lienart, M  Vanbellinghen, AM  Bolaky, N  Chan, CC  Caspers, L  Martin-Martinez, MD  Xu, H  Delporte, C  Willermain, F 
Citation: Motulsky E, etal., Mol Vis. 2010 Apr 3;16:602-10.
RGD ID: 5148029
Pubmed: PMID:20383338   (View Abstract at PubMed)
PMCID: PMC2850934   (View Article at PubMed Central)

PURPOSE: Blood-retinal barrier (BRB) breakdown and retinal edema are major complications of autoimmune uveitis and could be related to deregulation of aquaporin (AQP) expression. We have therefore evaluated the expression of AQP1 and AQP4 on BRB cells during experimental autoimmune uveitis (EAU) in mice. METHODS: C57Bl6 mice were immunized with interphotoreceptor retinoid-binding protein (IRBP) peptide 1-16. The disease was graded clinically, and double immunolabeling using glial fibrillary acidic protein (GFAP; a marker of disease activity) and AQP1 or AQP4 antibodies was performed at day 28. AQP1 expression was also investigated in mouse retinal pigment epithelium (RPE) cells (B6-RPE07 cell line) by reverse transcriptase PCR and western blot under basal and tumor necrosis factor alpha (TNF-alpha)-stimulated conditions. RESULTS: In both normal and EAU retina, AQP1 and AQP4 expression were restricted to the photoreceptor layer and to the Muller cells, respectively. Retinal endothelial cells never expressed AQP1. In vasculitis and intraretinal inflammatory infiltrates, decreased AQP1 expression was observed due to the loss of photoreceptors and the characteristic radial labeling of AQP4 was lost. On the other hand, no AQP4 expression was detected in RPE cells. AQP1 was strongly expressed by choroidal endothelial cells, rendering difficult the evaluation of AQP1 expression by RPE cells in vivo. No major differences were found between EAU and controls at this level. Interestingly, B6-RPE07 cells expressed AQP1 in vitro, and TNF-alpha downregulated AQP1 protein expression in those cells. CONCLUSIONS: Changes in retinal expression of AQP1 and AQP4 during EAU were primarily due to inflammatory lesions, contrasting with major modulation of AQP expression in BRB detected in other models of BRB breakdown. However, our data showed that TNF-alpha treatment strongly modulates AQP1 expression in B6-RPE07 cells in vitro.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
uveitis  ISOAqp1 (Rattus norvegicus)5148029; 5148029 RGD 
uveitis  ISOAqp4 (Rattus norvegicus)5148029; 5148029 RGD 
uveitis  IEP 5148029; 5148029 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aqp1  (aquaporin 1)
Aqp4  (aquaporin 4)

Genes (Mus musculus)
Aqp1  (aquaporin 1)
Aqp4  (aquaporin 4)

Genes (Homo sapiens)
AQP1  (aquaporin 1 (Colton blood group))
AQP4  (aquaporin 4)


Additional Information