RGD Reference Report - Function of interleukin-20 as a proinflammatory molecule in rheumatoid and experimental arthritis. - Rat Genome Database

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Function of interleukin-20 as a proinflammatory molecule in rheumatoid and experimental arthritis.

Authors: Hsu, YH  Li, HH  Hsieh, MY  Liu, MF  Huang, KY  Chin, LS  Chen, PC  Cheng, HH  Chang, MS 
Citation: Hsu YH, etal., Arthritis Rheum. 2006 Sep;54(9):2722-33.
RGD ID: 5147395
Pubmed: PMID:16947773   (View Abstract at PubMed)
DOI: DOI:10.1002/art.22039   (Journal Full-text)

OBJECTIVE: The pathogenesis of rheumatoid arthritis (RA) reflects an ongoing imbalance between proinflammatory and antiinflammatory cytokines. Interleukin-20 (IL-20) has proinflammatory properties for keratinocytes. In this study, we sought to determine whether IL-20 is involved in RA. METHODS: We analyzed IL-20 levels in synovial fluid from RA patients. IL-20 and its receptors were detected in RA synovial fibroblasts (RASFs), using immunohistochemical staining. The effect of IL-20 on endothelial cells, neutrophils, and RASFs was investigated using MTT and migration assays. The expression of IL-20 and its receptors in healthy rats and in rats with collagen-induced arthritis (CIA) was also analyzed. Soluble IL-20 receptor type I (sIL-20RI) or sIL-20RII was administered to rats with CIA by intramuscular electroporation, and the severity of arthritis was monitored. RESULTS: RA patients expressed significantly higher levels of synovial fluid IL-20 than did the rheumatic disease controls. IL-20 and its receptors were expressed in the synovial membranes and RASFs. IL-20 induced RASFs to secrete monocyte chemoattractant protein 1, IL-6, and IL-8, and it promoted neutrophil chemotaxis, RASF migration, and endothelial cell proliferation. Both IL-20 and IL-20RI were up-regulated in the rat CIA model. In vivo, electroporated sIL-20RI plasmid DNA decreased the severity of arthritis in the rats with CIA. CONCLUSION: IL-20 was up-regulated in the synovial fluid of RA patients and acted as a chemokine that attracted the migration of neutrophils and RASFs in vitro. The rat CIA model demonstrated that IL-20 was involved in the pathogenesis of arthritis, because sIL-20RI significantly reduced arthritis in rats with CIA. Thus, IL-20 may modulate the incidence and severity of arthritis and play important roles at local sites of inflammation.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
rheumatoid arthritis  IEP 5147395 RGD 
rheumatoid arthritis  ISOIL20 (Homo sapiens)5147395; 5147395 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il20  (interleukin 20)

Genes (Mus musculus)
Il20  (interleukin 20)

Genes (Homo sapiens)
IL20  (interleukin 20)


Additional Information