RGD Reference Report - Differential changes in the concentrations of cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-2 in exudate during rat lipopolysaccharide-induced inflammation. - Rat Genome Database

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Differential changes in the concentrations of cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-2 in exudate during rat lipopolysaccharide-induced inflammation.

Authors: Shibata, F  Kato, H  Konishi, K  Okumora, A  Ochiai, H  Nakajima, K  Al-Mokdad, M  Nakagawa, H 
Citation: Shibata F, etal., Cytokine. 1996 Mar;8(3):222-6.
RGD ID: 5135240
Pubmed: PMID:8833037   (View Abstract at PubMed)

Recently we have purified four neutrophil chemotactic factors from conditioned medium of rat granulation tissue. Two chemokines besides cytokine-induced neutrophil chemoattractant-1 (CINC-1, formerly called CINC) and CNC-3/macrophage inflammatory protein-2 (MIP-2) were novel chemoattractants, designated as CINC-2 alpha and CINC-2 beta. In the present report, we developed an enzyme-linked immunosorbent assay (ELISA) specific for CINC-2. The biotin-streptavidin sandwich ELISA for CINC-2 beta detected CINC-2 alpha and CINC- 2 beta equally well between 1 ug/mI and 300 ng/ml, but did not show cross-reactivity with CINC-1 and CINC-3. The concentrations of CINC-1 and CINC-2 in the exudate during rat lipopolysaccharide (LPS)-induced inflammation were determined. The CINC-1 concentration in the exudate reached a maximum at 4 h after LPS injection, whereas the CINC-2 level steadily increased up to 8 h. The number of infiltrated cells in the exudate increased linearly until 6 h and gradually up to 8 h. Increase in the cell number was correlated with total concentrations of CINC-1 and CINC-2. The results suggest that CINC-2 as well as CINC-1 plays an important role in accumulation of neutrophils into the inflammatory lesion of LPS- induced inflammation in rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Inflammation  ISOCxcl1 (Rattus norvegicus)5135240; 5135240 RGD 
Inflammation  ISOCxcl3 (Rattus norvegicus)5135240; 5135240 RGD 
Inflammation  IEP 5135240; 5135240 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl1  (C-X-C motif chemokine ligand 1)
Cxcl3  (C-X-C motif chemokine ligand 3)

Genes (Mus musculus)
Cxcl1  (C-X-C motif chemokine ligand 1)
Cxcl3  (C-X-C motif chemokine ligand 3)

Genes (Homo sapiens)
CXCL1  (C-X-C motif chemokine ligand 1)
CXCL3  (C-X-C motif chemokine ligand 3)


Additional Information