RGD Reference Report - Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.

General

Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.
Authors:	Treuter, E Johansson, L Thomsen, JS Warnmark, A Leers, J Pelto-Huikko, M Sjoberg, M Wright, AP Spyrou, G Gustafsson, JA
Citation:	Treuter E, etal., J Biol Chem. 1999 Mar 5;274(10):6667-77.
RGD ID:	5134969
Pubmed:	PMID:10037764 (View Abstract at PubMed)
Transcriptional activation by nuclear receptors (NRs) involves the concerted action of coactivators, chromatin components, and the basal transcription machinery. Crucial NR coactivators, which target primarily the conserved ligand-regulated activation (AF-2) domain, include p160 family members, such as TIF2, as well as p160-associated coactivators, such as CBP/p300. Because these coactivators possess intrinsic histone acetyltransferase activity, they are believed to function mainly by regulating chromatin-dependent transcriptional activation. Recent evidence suggests the existence of an additional NR coactivator complex, referred to as the thyroid hormone receptor-associated protein (TRAP) complex, which may function more directly as a bridging complex to the basal transcription machinery. TRAP220, the 220-kDa NR-binding subunit of the complex, has been identified in independent studies using both biochemical and genetic approaches. In light of the functional differences identified between p160 and TRAP coactivator complexes in NR activation, we have attempted to compare interaction and functional characteristics of TIF 2 and TRAP220. Our findings imply that competition between the NR-binding subunits of distinct coactivator complexes may act as a putative regulatory step in establishing either a sequential activation cascade or the formation of independent coactivator complexes.

Gene Ontology Annotations Click to see Annotation Detail View

Biological Process

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
positive regulation of transcription by RNA polymerase II		IDA		5134969		RGD

Cellular Component

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
protein-DNA complex		IDA		5134969		RGD

Molecular Function

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
general transcription initiation factor binding		IPI	TBP (Homo sapiens)	5134969		RGD
histone acetyltransferase binding		IPI	Crebbp (Mus musculus)	5134969		RGD
nuclear thyroid hormone receptor binding		IDA		5134969		RGD
nuclear thyroid hormone receptor binding		IPI	THRA (Homo sapiens)	5134969		RGD
peroxisome proliferator activated receptor binding		IPI	Pparg (Rattus norvegicus)	5134969		RGD
protein-containing complex binding		IDA		5134969		RGD
transcription coactivator binding		IPI	Med1 (Rattus norvegicus)	5134969		RGD
transcription coregulator activity		IDA		5134969		RGD

Objects Annotated

Genes (Rattus norvegicus)

Med1 (mediator complex subunit 1)

Pparg (peroxisome proliferator-activated receptor gamma)

Rxra (retinoid X receptor alpha)

Additional Information

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InterViewer (Protein-Protein Interactions)	GViewer (Genome Viewer)	Variant Visualizer (Damaging Variants)

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Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.