RGD Reference Report - Genes of the antioxidant response undergo upregulation in a rodent model of nonalcoholic steatohepatitis. - Rat Genome Database

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Genes of the antioxidant response undergo upregulation in a rodent model of nonalcoholic steatohepatitis.

Authors: Lickteig, AJ  Fisher, CD  Augustine, LM  Cherrington, NJ 
Citation: Lickteig AJ, etal., J Biochem Mol Toxicol. 2007;21(4):216-20.
RGD ID: 5133249
Pubmed: PMID:17721935   (View Abstract at PubMed)
DOI: DOI:10.1002/jbt.20177   (Journal Full-text)

Nonalcoholic fatty liver disease encompasses a spectrum of hepatic pathologies ranging from simple fatty liver to an inflammatory state known as nonalcoholic steatohepatitis (NASH). NASH is also characterized by severe hepatic oxidative stress. The goal of this study was to determine whether genes of the antioxidant response are induced in rodent models of nonalcoholic fatty liver disease. To simulate simple fatty liver and NASH, respectively, male Sprague-Dawley rats were fed a high-fat (HF) or a methionine and choline-deficient (MCD) diet for 8 weeks. Key marker genes of the antioxidant response that are known to undergo upregulation via activation of Nuclear Factor Erythroid 2-Related Factor 2 were measured using the branched DNA signal amplification assay. Messenger RNA levels of the antioxidant response, including NAD(P)H:quinone oxidoreductase-1 (Nqo1), Glutamate cysteine ligase catalytic (Gclc), and Heme oxygenase-1 (Ho-1), were significantly induced in MCD rat liver but not in HF rat liver. Furthermore, Nqo1 protein expression and activity underwent significant upregulation in MCD rat liver but not in HF rat liver. These data strongly indicate that the pathology induced by the MCD dietary model of NASH results in upregulation of the antioxidant response in rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
steatotic liver disease  ISONqo1 (Rattus norvegicus)5133249; 5133249protein:increased activity:liverRGD 
steatotic liver disease  IEP 5133249protein:increased activity:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Nqo1  (NAD(P)H quinone dehydrogenase 1)

Genes (Mus musculus)
Nqo1  (NAD(P)H dehydrogenase, quinone 1)

Genes (Homo sapiens)
NQO1  (NAD(P)H quinone dehydrogenase 1)


Additional Information