RGD Reference Report - Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis. - Rat Genome Database

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Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis.

Authors: Wong, EW  Mruk, DD  Lee, WM  Cheng, CY 
Citation: Wong EW, etal., Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9657-62. Epub 2008 Jul 10.
RGD ID: 5131983
Pubmed: PMID:18621709   (View Abstract at PubMed)
PMCID: PMC2474483   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0801527105   (Journal Full-text)

The Par3/Par6/aPKC and the CRB3/Pals1/PATJ polarity complexes are involved in regulating apical ectoplasmic specialization (ES) and blood-testis barrier (BTB) restructuring in the testis. Par6 was a component of the apical ES and the BTB. However, its level was considerably diminished at both sites at stage VIII of the cycle. Par6 also formed a stable complex with Pals1 and JAM-C (a component of the apical ES) in normal testes. When rats were treated with adjudin to induce apical ES restructuring without compromising the BTB, Par6 staining virtually disappeared at the apical ES in misaligned spermatids before their depletion. Additionally, the Par6/Pals1 complex became tightly associated with Src kinase, rendering a loss of association of the Par6/Pals1 complex with JAM-C, thereby destabilizing apical ES to facilitate spermatid loss. Primary Sertoli cell cultures with established functional BTB, but without apical ES, were next used to assess the Par6-based complex on BTB dynamics. When either Par6 or Par3 was knocked down by RNAi in Sertoli cell epithelium, a significant loss of the corresponding protein by approximately 60% in cells vs. controls was detected, alongside with a decline in aPKC after Par6, but not Par3, knockdown. This Par3 or Par6 knockdown also led to a transient loss of selected BTB proteins at the cell-cell interface, thereby compromising the BTB integrity. These findings illustrate that the Par6/Par3-based polarity complex likely coordinates the events of apical ES and BTB restructuring that take place concurrently at the opposing ends of adjacent Sertoli cells in the seminiferous epithelium during spermatogenesis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
regulation of cellular localization  IMP 5131983; 5131983 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex  IDA 5131983; 5131983; 5131983; 5131983; 5131983; 5131983; 5131983 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Crb3  (crumbs cell polarity complex component 3)
Pals1  (protein associated with LIN7 1, MAGUK p55 family member)
Pard3  (par-3 family cell polarity regulator)
Pard6a  (par-6 family cell polarity regulator alpha)
Pard6b  (par-6 family cell polarity regulator beta)
Pard6g  (par-6 family cell polarity regulator gamma)
Patj  (PATJ, crumbs cell polarity complex component)


Additional Information