RGD Reference Report - In vitro and in vivo induction and activation of nNOS by LPS in oligodendrocytes. - Rat Genome Database

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In vitro and in vivo induction and activation of nNOS by LPS in oligodendrocytes.

Authors: Yao, SY  Ljunggren-Rose, A  Chandramohan, N  Whetsell WO, JR  Sriram, S 
Citation: Yao SY, etal., J Neuroimmunol. 2010 Dec 15;229(1-2):146-56. Epub 2010 Aug 19.
RGD ID: 5131926
Pubmed: PMID:20724006   (View Abstract at PubMed)
PMCID: PMC5362965   (View Article at PubMed Central)
DOI: DOI:10.1016/j.jneuroim.2010.07.023   (Journal Full-text)

There are currently four known isoforms of nitric oxide synthase (NOS). Of these, neuronal NOS (nNOS) is known to be present exclusively in neurons, endothelial NOS (eNOS) in vascular endothelium, while the inducible form of NOS (iNOS) is known to be activated in oligodendrocytes, astrocytes and microglia. The fourth isoform, mitochondrial NOS (mtNOS), represents a post-translational modification of nNOS. Using western blotting and real time-PCR, we show induction and activation of nNOS following culture of oligodendrocyte progenitor cells (OPC) with lipopolysaccharide (LPS). Activation of nNOS results in accumulation of peroxynitrite and tyrosine nitration of proteins in oligodendrocytes resulting in reduced cell viability. Injection of LPS in vivo into the corpus callosum of rats leads to the development of extensive demyelination of the white matter tracts. Immunostaining of regions close to the injection site shows the presence of nNOS, but not iNOS, in oligodendrocytes. Neither iNOS nor nNOS was seen in astrocytes in areas of demyelination. These studies suggest that activation of nNOS in oligodendrocytes leads to oligodendrocyte injury resulting in demyelination.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to lipopolysaccharide  IEP 5131926 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nos1  (nitric oxide synthase 1)


Additional Information