RGD Reference Report - Expression changes of dopaminergic system-related genes in PC12 cells induced by manganese, silver, or copper nanoparticles. - Rat Genome Database

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Expression changes of dopaminergic system-related genes in PC12 cells induced by manganese, silver, or copper nanoparticles.

Authors: Wang, J  Rahman, MF  Duhart, HM  Newport, GD  Patterson, TA  Murdock, RC  Hussain, SM  Schlager, JJ  Ali, SF 
Citation: Wang J, etal., Neurotoxicology. 2009 Nov;30(6):926-33. Epub 2009 Sep 23.
RGD ID: 5130971
Pubmed: PMID:19781568   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuro.2009.09.005   (Journal Full-text)

Nanoparticles have received a great deal of attention for producing new engineering applications due to their novel physicochemical characteristics. However, the broad application of nanomaterials has also produced concern for nanoparticle toxicity due to increased exposure from large-scale industry production. This study was conducted to investigate the potential neurotoxicity of manganese (Mn), silver (Ag), and copper (Cu) nanoparticles using the dopaminergic neuronal cell line, PC12. Selective genes associated with the dopaminergic system were investigated for expression changes and their correlation with dopamine depletion. PC12 cells were treated with 10 microg/ml Mn-40 nm, Ag-15 nm, or Cu-90 nm nanoparticles for 24 h. Cu-90 nanoparticles induced dopamine depletion in PC12 cells, which is similar to the effect induced by Mn-40 shown in a previous study. The expression of 11 genes associated with the dopaminergic system was examined using real-time RT-PCR. The expression of Txnrd1 was up-regulated after the Cu-90 treatment and the expression of Gpx1 was down-regulated after Ag-15 or Cu-90 treatment. These alterations are consistent with the oxidative stress induced by metal nanoparticles. Mn-40 induced a down-regulation of the expression of Th; Cu-90 induced an up-regulation of the expression of Maoa. This indicates that besides the oxidation mechanism, enzymatic alterations may also play important roles in the induced dopamine depletion. Mn-40 also induced a down-regulation of the expression of Park2; while the expression of Snca was up-regulated after Mn-40 or Cu-90 treatment. These data suggest that Mn and Cu nanoparticles-induced dopaminergic neurotoxicity may share some common mechanisms associated with neurodegeneration.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to copper ion  IEP 5130971 RGD 
cellular response to manganese ion  IEP 5130971; 5130971 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prkn  (parkin RBR E3 ubiquitin protein ligase)
Th  (tyrosine hydroxylase)
Txnrd1  (thioredoxin reductase 1)


Additional Information