RGD Reference Report - Rotavirus double-stranded RNA induces apoptosis and diminishes wound repair in rat intestinal epithelial cells. - Rat Genome Database

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Rotavirus double-stranded RNA induces apoptosis and diminishes wound repair in rat intestinal epithelial cells.

Authors: Sato, A  Iizuka, M  Nakagomi, O  Suzuki, M  Horie, Y  Konno, S  Hirasawa, F  Sasaki, K  Shindo, K  Watanabe, S 
Citation: Sato A, etal., J Gastroenterol Hepatol. 2006 Mar;21(3):521-30.
RGD ID: 5128794
Pubmed: PMID:16638093   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1440-1746.2005.03977.x   (Journal Full-text)

BACKGROUND: Recent studies have shown that toll-like receptor 3 (TLR3) recognizes double-stranded RNA (dsRNA). Rotaviruses, having a dsRNA genome, infect intestinal epithelial cells (IEC) and cause acute gastroenteritis in young children. The aim of the present study was to clarify the pathophysiological function of rotavirus dsRNA in IEC. METHODS: Expression of TLR3 mRNA or protein in IEC cell lines (IEC-6, HT-29, Caco-2) was assessed by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis or immunohistochemistry. Induction of cytokines (TNF-alpha, interferon-beta, interleukin-6) mRNA and activation of signal proteins (ERK1/2 MAPK and IkappaB-alpha) in IEC after stimulation with rotavirus dsRNA were assessed by RT-PCR or Western blot analysis. IEC-6 cells were wounded and cell migration into wound areas after stimulation with rotavirus dsRNA (1-25 microg/mL) was assessed. Induction of apoptosis after stimulation with rotavirus dsRNA was also assessed. RESULTS: Expression of TLR3 mRNA and TLR3 protein was detected in IEC. Expression of TLR3 mRNA in IEC-6 tended to be up-regulated by exposure to IFN-gamma. Induction of cytokine mRNA and activation of the signal proteins were detected after stimulation with rotavirus dsRNA. Apoptosis was induced and epithelial migration into the wound area was dose-dependently diminished (44.1-94.4%, P < 0.01) by exposure to rotavirus dsRNA. Diminishment of wound repair was suppressed by anti-TLR3 antibody or caspase inhibitor. Conclusion: Rotavirus dsRNA induces severe apoptosis and diminishes wound repair in IEC through TLR3, which might be involved in the pathogenesis of rotavirus-induced enteritis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to type II interferon  IEP 5128794intestinal epithelial cellsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Tlr3  (toll-like receptor 3)


Additional Information