RGD Reference Report - In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. - Rat Genome Database

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In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection.

Authors: Kurland, AR  Schreiner, H  Diamond, G 
Citation: Kurland AR, etal., J Periodontal Res. 2006 Dec;41(6):567-72.
RGD ID: 4892269
Pubmed: PMID:17076783   (View Abstract at PubMed)
PMCID: PMC2094111   (View Article at PubMed Central)
DOI: DOI:10.1111/j.1600-0765.2006.00909.x   (Journal Full-text)

BACKGROUND AND OBJECTIVE: Human beta-defensins have been identified in the oral cavity and are predicted to play a role in the defense against pathogenic bacteria. Homologous rat beta-defensins (RBDs) have been identified, but their expression in the oral cavity has not been examined. Therefore, the aim of this study was to investigate the expression of innate immune mediators in the rat gingival epithelium. MATERIAL AND METHODS: Rats were pretreated with antibiotics to depress the normal oral flora, followed by the introduction of Actinobacillus actinomycetemcomitans in their food to allow colonization and the development of periodontal disease. At various time points, animals were killed and the gingival epithelium was extracted. Semiquantitative reverse transcription-polymerase chain reaction was performed to measure RBD and Toll-like receptor (TLR) mRNA levels. RESULTS: Three beta-defensins (RBD-1, -2 and -5) and two TLRs (TLR-3 and -4) are expressed in normal rat gingival epithelium. After the introduction of A. actinomycetemcomitans, RBD-1 and RBD-2 mRNA levels increased for the first week followed by a return to basal levels. No change in TLR mRNA levels was observed. CONCLUSION: The rat model provides a good system for experimental analysis of the innate immune response to periopathogenic bacteria in the oral cavity, as well as the potential role of beta-defensins in the host response to colonization.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Actinobacillus Infections  ISODefb1 (Rattus norvegicus)4892269; 4892269mRNA:increased expression:gingival epitheliumRGD 
Actinobacillus Infections  IEP 4892269mRNA:increased expression:gingival epitheliumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Defb1  (defensin beta 1)

Genes (Mus musculus)
Defb1  (defensin beta 1)

Genes (Homo sapiens)
DEFB1  (defensin beta 1)


Additional Information