RGD Reference Report - Functional and phenotypic characteristics of testicular macrophages in experimental autoimmune orchitis. - Rat Genome Database

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Functional and phenotypic characteristics of testicular macrophages in experimental autoimmune orchitis.

Authors: Rival, C  Theas, MS  Suescun, MO  Jacobo, P  Guazzone, V  Van Rooijen, N  Lustig, L 
Citation: Rival C, etal., J Pathol. 2008 Jun;215(2):108-17.
RGD ID: 4892229
Pubmed: PMID:18381617   (View Abstract at PubMed)
DOI: DOI:10.1002/path.2328   (Journal Full-text)

Testicular inflammation with compromised fertility can occur despite the fact that the testis is considered an immunoprivileged organ. Testicular macrophages have been described as cells with an immunosuppressor profile, thus contributing to the immunoprivilege of the testis. Experimental autoimmune orchitis (EAO) is a model of organ-specific autoimmunity and testicular inflammation. EAO is characterized by an interstitial inflammatory mononuclear cell infiltration, damage of the seminiferous tubules and germ cell apoptosis. Here we studied the phenotype and functions of testicular macrophages during the development of EAO. By stereological analysis, we detected an increased number of resident (ED2+) and non-resident (ED1+) macrophages in the testicular interstitium of rats with orchitis. We showed that this increase was mainly due to monocyte recruitment. The in vivo administration of liposomes containing clodronate in rats undergoing EAO led to a reduction in the number of testicular macrophages, which correlated with a decreased incidence and severity of the testicular damage and suggests a pathogenic role of macrophages in EAO. By immunohistochemistry and flow cytometry we detected an increased number of testicular macrophages expressing MHC class II, CD80 and CD86 costimulatory molecules in rats with orchitis. Also, testicular macrophages from rats with EAO showed a higher production of IFNgamma (ELISA). We conclude that testicular macrophages participate in EAO development, and the ED1+ macrophage subset is the main pathogenic subpopulation. They stimulate the immune response through the production of pro-inflammatory cytokines and antigen presentation and thus activation of T cells in the target organ.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
orchitis  ISOCd86 (Rattus norvegicus)4892229; 4892229protein:increased expression:testis and macrophage (rat)RGD 
orchitis  IEP 4892229protein:increased expression:testis and macrophage (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cd86  (CD86 molecule)

Genes (Mus musculus)
Cd86  (CD86 antigen)

Genes (Homo sapiens)
CD86  (CD86 molecule)


Additional Information