RGD Reference Report - Human circulating eosinophils secrete macrophage migration inhibitory factor (MIF). Potential role in asthma. - Rat Genome Database

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Human circulating eosinophils secrete macrophage migration inhibitory factor (MIF). Potential role in asthma.

Authors: Rossi, AG  Haslett, C  Hirani, N  Greening, AP  Rahman, I  Metz, CN  Bucala, R  Donnelly, SC 
Citation: Rossi AG, etal., J Clin Invest. 1998 Jun 15;101(12):2869-74.
RGD ID: 4891043
Pubmed: PMID:9637721   (View Abstract at PubMed)
PMCID: PMC508878   (View Article at PubMed Central)
DOI: DOI:10.1172/JCI1524   (Journal Full-text)

Macrophage migration inhibitory factor (MIF) is a potent proinflammatory mediator that has been shown to potentiate lethal endotoxemia and to play a potentially important regulatory role in human acute respiratory distress syndrome (ARDS). We have investigated whether eosinophils are an important source of MIF and whether MIF may be involved in the pathophysiology of asthma. Unstimulated human circulating eosinophils were found to contain preformed MIF. Stimulation of human eosinophils with phorbol myristate acetate in vitro yielded significant release of MIF protein. For example, eosinophils stimulated with phorbol myristate acetate (100 nM, 8 h, 37 degreesC) released 1,539+/-435 pg/10(6) cells of MIF, whereas unstimulated cells released barely detectable levels (< 142 pg/10(6) cells, mean+/-SEM, n = 8). This stimulated release was shown to be (a) concentration- and time-dependent, (b) partially blocked by the protein synthesis inhibitor cycloheximide, and (c) significantly inhibited by the protein kinase C inhibitor Ro-31,8220. In addition, we show that the physiological stimuli C5a and IL-5 also cause significant MIF release. Furthermore, bronchoalveolar lavage fluid obtained from asthmatic patients contains significantly elevated levels of MIF as compared to nonatopic normal volunteers (asthmatic, 797.5+/-92 pg/ml; controls, 274+/-91 pg/ml). These results highlight the potential importance of MIF in asthma and other eosinophil-dependent inflammatory disorders.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
asthma  IEP 4891043protein:increased expression:bronchoalveolar lavage fluidRGD 
asthma  ISOMIF (Homo sapiens)4891043; 4891043protein:increased expression:bronchoalveolar lavage fluidRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mif  (macrophage migration inhibitory factor)

Genes (Mus musculus)
Mif  (macrophage migration inhibitory factor (glycosylation-inhibiting factor))

Genes (Homo sapiens)
MIF  (macrophage migration inhibitory factor)


Additional Information