RGD Reference Report - Hyperoxia increases protein mass of 5-lipoxygenase and its activating protein, flap, and leukotriene B(4) output in newborn rat lungs. - Rat Genome Database

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Hyperoxia increases protein mass of 5-lipoxygenase and its activating protein, flap, and leukotriene B(4) output in newborn rat lungs.

Authors: Hosford, GE  Koyanagi, KS  Leung, WI  Olson, DM 
Citation: Hosford GE, etal., Exp Lung Res. 2002 Dec;28(8):671-84.
RGD ID: 4890420
Pubmed: PMID:12490039   (View Abstract at PubMed)
DOI: DOI:10.1080/01902140260426751   (Journal Full-text)

In this study, the authors examined in newborn rat lung tissues the release of leukotriene B(4) (LTB(4)) from tissue explants in vitro, the protein expression of the LT-synthesizing enzyme, 5-lipoxygenase (5-LO), and its activating protein (FLAP), and the effects of in vivo hyperoxic exposure (>95% O(2)) on these parameters. Basal LTB(4) output increased from 0.98 ng/mgDNA/30 min at day 1 to 3.3 ng/mgDNA/30 min at day 28 (P <.05). Exposure of rat pups to >95% O(2) from days 1 to 7 and 60% O(2) from days 7 to 28 stimulated a 1.6-fold (P <.05) increase in LTB(4) output, compared to normoxic pups (to 1.6 ng/mgDNA/30 min) by day 1 and on day 7. The calcium ionophore, A23187, caused an increase in LTB(4) output from both exposure groups, but LTB(4) output was consistently greater (P <.05) from hyperoxia-exposed pups. Western immunoblotting of lung tissue showed that 5-LO and FLAP protein mass increased (P <.05) from days 4 to 14. Hyperoxia exposure increased the mass of both proteins (P <.05). Immunohistochemistry localized 5-LO and FLAP mainly to alveolar macrophages on day 14, but some staining was evident in parenchymal tissue. These data show that hyperoxia increases LTB(4) output, as well as protein levels of 5-LO and FLAP, in newborn rat lungs during early postnatal life. Elevated LTB(4) may contribute to the etiology of newborn lung disease.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Hyperoxia  ISOAlox5 (Rattus norvegicus)4890420; 4890420protein:increased expression:lungRGD 
Hyperoxia  ISOAlox5ap (Rattus norvegicus)4890420; 4890420protein:increased expression:lungRGD 
Hyperoxia  IEP 4890420; 4890420protein:increased expression:lungRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to hyperoxia  IEP 4890420 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Alox5  (arachidonate 5-lipoxygenase)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Mus musculus)
Alox5  (arachidonate 5-lipoxygenase)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Homo sapiens)
ALOX5  (arachidonate 5-lipoxygenase)
ALOX5AP  (arachidonate 5-lipoxygenase activating protein)


Additional Information