RGD Reference Report - Differential effects of nonylphenol on testosterone secretion in rat Leydig cells. - Rat Genome Database

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Differential effects of nonylphenol on testosterone secretion in rat Leydig cells.

Authors: Wu, JJ  Wang, KL  Wang, SW  Hwang, GS  Mao, IF  Chen, ML  Wang, PS 
Citation: Wu JJ, etal., Toxicology. 2010 Jan 31;268(1-2):1-7. Epub 2009 Oct 31.
RGD ID: 4145609
Pubmed: PMID:19883722   (View Abstract at PubMed)
DOI: DOI:10.1016/j.tox.2009.10.030   (Journal Full-text)

Nonylphenol (NP), a final metabolite of nonylphenol polyethoxylate, has been reported to interfere with male reproduction. However, its mechanisms are not fully understood. In the present study, we examined the effects of NP on steroidogenesis of testosterone in rat Leydig cells. The testosterone concentrations in rat plasma were examined after intravenous injection of NP (100 microg/kg) at different time intervals. In addition, rat Leydig cells were challenged with different concentrations of NP (4.25-127.5 microM) to evaluate its influences on testosterone steroidogenesis. Administration of NP showed a decrease of hCG-induced plasma testosterone. Moreover, in vitro experiments revealed that NP (127.5 microM) alone stimulated testosterone release through increase of both protein levels and activities of the StAR and P450(SCC). In contrast, NP inhibited hCG-induced testosterone release in rat Leydig cells. The inhibitory effect was also observed after incubation of the Leydig cells in the presence of different precursors. These results suggested that NP had differential effects on testosterone synthesis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
testosterone biosynthetic process  IEP 4145609; 4145609 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp11a1  (cytochrome P450, family 11, subfamily a, polypeptide 1)
Star  (steroidogenic acute regulatory protein)


Additional Information