RGD Reference Report - [The effect of cigarette smoke on the expression of transforming growth factor-beta1 mRNA and collagen type III in airways of asthmatic rats] - Rat Genome Database

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[The effect of cigarette smoke on the expression of transforming growth factor-beta1 mRNA and collagen type III in airways of asthmatic rats]

Authors: Guo, ZH  DU, YC  Xu, JY 
Citation: Guo ZH, etal., Zhonghua Jie He He Hu Xi Za Zhi. 2008 Jan;31(1):42-5.
RGD ID: 4145280
Pubmed: PMID:18366906   (View Abstract at PubMed)

OBJECTIVE: To study the effect of cigarette smoke on the expression of transforming growth factor-beta l (TGF-beta1), and collagen type III in lung tissues, and therefore to investigate the mechanism of airway remodeling. METHODS: Thirty male Wistar rats were randomly divided into a control group, an asthmatic group and a cigarette smoke treated group, with 10 rats in each group. The expression level of TGF-beta1 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) and collagen type III by immunohistochemistry. The thickness of airway wall in each group was also measured. Software SPSS 11.0 was used for statistical analysis (data expressed as +/- s). Group comparison was made by one way ANOVA and Pearson correlation was used for correlation analysis. RESULTS: The levels of TGF-beta1 mRNA and collagen type III in cigarette smoke treated group (0.42 +/- 0.04, 25.8 +/- 2.3) were higher than those in the asthmatic group (0.39 +/- 0.04, 22.9 +/- 3.1) and in the control group (0.26 +/- 0.04, 16.3 +/- 2.3). Compared to the control group, the levels were higher in the asthmatic group (F = 55.97, 35.61, all P < 0.05). The level of TGF-beta1 mRNA was positively correlated with the expression of collagen type III (r = 0.71, P < 0.05). The thickness of airway wall in the cigarette smoke treated group (23.3 +/- 2.4) microm2/microm was significantly higher than that in the asthmatic group (20.1 +/- 2.9) microm2/microm and the control group (11.6 +/- 2.4) microm2/microm;compared to the control group, it was higher in the asthmatic group (F = 53.68, P < 0.05). CONCLUSION: Cigarette smoke can promote over-expression of TGF-beta1-mRNA in asthmatic rat airways, increase the expression of collagen type III and aggravate airway remodeling.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
asthma  ISOTgfb1 (Rattus norvegicus)4145280; 4145280 RGD 
asthma  IEP 4145280 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tgfb1  (transforming growth factor, beta 1)

Genes (Mus musculus)
Tgfb1  (transforming growth factor, beta 1)

Genes (Homo sapiens)
TGFB1  (transforming growth factor beta 1)


Additional Information