RGD Reference Report - Blunt chest trauma induces mediator-dependent monocyte migration to the lung. - Rat Genome Database

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Blunt chest trauma induces mediator-dependent monocyte migration to the lung.

Authors: Seitz, DH  Niesler, U  Palmer, A  Sulger, M  Braumuller, ST  Perl, M  Gebhard, F  Knoferl, MW 
Citation: Seitz DH, etal., Crit Care Med. 2010 Sep;38(9):1852-9.
RGD ID: 4144894
Pubmed: PMID:20543668   (View Abstract at PubMed)
DOI: DOI:10.1097/CCM.0b013e3181e8ad10   (Journal Full-text)

OBJECTIVE: This study was designed to determine whether lung contusion induces an increased pulmonary recruitment of monocytes as a source of alveolar macrophages and which mediators are involved. SETTING AND DESIGN: Prospective animal study. SUBJECTS AND INTERVENTIONS: Male Sprague-Dawley rats were subjected to chest trauma by a single blast wave. MEASUREMENTS: Chemokine concentrations in bronchoalveolar lavage fluids and supernatants of alveolar macrophages, chemokine and chemokine receptor mRNA expressions in monocytes, pulmonary interstitial macrophages, and alveolar macrophages isolated after trauma or sham procedure were evaluated. Immigration of monocytes was determined by staining alveolar macrophages with the fluorescent marker PKH26 before chest trauma. Chemotaxis of naive monocytes in response to bronchoalveolar lavage or supernatants from alveolar macrophages isolated after trauma or sham procedure and the migratory response of monocytes isolated after trauma/sham to recombinant chemokines were measured. MAIN RESULTS: Chemokine levels in bronchoalveolar lavage and alveolar macrophage supernatants and the percentage of monocytes migrated to the lungs were increased after chest trauma. Lung contusion enhanced the mRNA expression for CCR2 in monocytes and interstitial macrophages and for monocyte chemotactic protein-1 in alveolar macrophages. Migration of naive monocytes vs. bronchoalveolar lavage or alveolar macrophage supernatants from traumatized animals was increased when compared with samples from shams. Monocytes isolated 2 hrs after trauma showed a reduced migration to CINC-1 or monocyte chemotactic protein-1 compared with sham. CONCLUSIONS: Alveolar macrophages seem to contribute to increased chemokine concentrations in alveoli of animals subjected to blunt chest trauma. Mediators released by alveolar macrophage are potent stimuli for monocyte migration. Monocytes alter their chemokine receptor expression and are recruited to the lungs.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Thoracic Injuries  ISOCcr2 (Rattus norvegicus)4144894; 4144894 RGD 
Thoracic Injuries  IEP 4144894 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccr2  (C-C motif chemokine receptor 2)

Genes (Mus musculus)
Ccr2  (C-C motif chemokine receptor 2)

Genes (Homo sapiens)
CCR2  (C-C motif chemokine receptor 2)


Additional Information