RGD Reference Report - Danshensu protects vascular endothelia in a rat model of hyperhomocysteinemia. - Rat Genome Database

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Danshensu protects vascular endothelia in a rat model of hyperhomocysteinemia.

Authors: Yang, RX  Huang, SY  Yan, FF  Lu, XT  Xing, YF  Liu, Y  Liu, YF  Zhao, YX 
Citation: Yang RX, etal., Acta Pharmacol Sin. 2010 Oct;31(10):1395-400. Epub 2010 Sep 27.
RGD ID: 4144131
Pubmed: PMID:20871618   (View Abstract at PubMed)
PMCID: PMC4085698   (View Article at PubMed Central)
DOI: DOI:10.1038/aps.2010.167   (Journal Full-text)

Aim:To examine whether danshensu could protect vascular endothelia in a rat model of hyperhomocysteinemia.Methods:The model was established by feeding rats with a methionine-rich diet (1 g.kg(-1).d(-1)) for 3 months. Immediately following the discontinuation of methionine-rich diet, rats were treated with danshensu (67.5 mg.kg(-1).d(-1), po) or saline for 3 additional months. One group of rats receiving vitamin mixture (folic acid, vitamin B12 and vitamin B6) was included as a positive control. One group of rats not exposed to methionine-rich diet was also included as a blank control. The expression of tumor necrosis factor-alpha (TNF-alpha) and intercellular adhesion molecule-1 (ICAM-1) protein in the descending aorta was examined using immunohistochemistry and Western blot. Homocysteine and blood concentration of endothelin and nitric oxide (NO) was also examined.Results:Methionine-rich diet resulted in accumulation of "foam cells", up-regulated expression of TNF-alpha and ICAM-1 in the descending aorta, and significantly increased serum homocysteine. Plasma endothelin concentration was significantly increased; NO was decreased. Danshensu treatment, either simultaneous to methionine-rich diet or afterwards, attenuated the above mentioned changes.Conclusion:Chronic treatment with danshensu could prevent/attenuate the formation of atherosclerosis. Potential mechanisms include inhibited expression of representative proinflammatory cytokines and adhesion molecules in arterial endothelia. Changes in homocysteine and circulating molecules that control vascular contraction/relaxation via endothelial cells (eg, endothelin and NO) were also implicated.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hyperhomocysteinemia  ISOIcam1 (Rattus norvegicus)4144131; 4144131protein:increased expression:descending aorta and endothelial cells (rat)RGD 
hyperhomocysteinemia  IEP 4144131protein:increased expression:descending aorta and endothelial cells (rat)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to amino acid  IEP 4144131methionine-rich dietRGD 

Objects Annotated

Genes (Rattus norvegicus)
Edn1  (endothelin 1)
Icam1  (intercellular adhesion molecule 1)

Genes (Mus musculus)
Icam1  (intercellular adhesion molecule 1)

Genes (Homo sapiens)
ICAM1  (intercellular adhesion molecule 1)


Additional Information