RGD Reference Report - Expression and localization of Fas-associated proteins following focal cerebral ischemia in rats. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Expression and localization of Fas-associated proteins following focal cerebral ischemia in rats.

Authors: Bi, FF  Xiao, B  Hu, YQ  Tian, FF  Wu, ZG  Ding, L  Zhou, XF 
Citation: Bi FF, etal., Brain Res. 2008 Jan 29;1191:30-8. Epub 2007 Nov 12.
RGD ID: 4142863
Pubmed: PMID:18096138   (View Abstract at PubMed)
DOI: DOI:10.1016/j.brainres.2007.10.098   (Journal Full-text)

The aim of this study was to investigate the changes of expression of Fas-associated proteins and its cellular localization in the peri-infarct region following transient focal cerebral ischemia. Adult male Sprague-Dawley rats underwent right middle cerebral artery occlusion (MCAo) for 2 h and reperfusion for 1, 3, 6, 12 and 24 h. The expression of Fas-associated death domain protein (FADD), Fas-associated phosphatase-1 (FAP-1) caspase-8 and death-associated protein (Daxx), the pro-apoptotic genes, were examined by methods of RT-PCR, immunohistochemistry and Western blot. The results showed that the expression levels of mRNA and protein for FADD and caspase-8 increased significantly at 1-3 h after reperfusion, peaked at 12 h, then declined markedly at 24 h. The time course change of FAP-1 was consistent with that of FADD. The expression level of mRNA and protein for death-associated protein (Daxx) increased significantly at 3 h after reperfusion and persisted for 24 h at a high level. Immunofluorescence double-staining laser scanning showed that the immunoreactivity of FADD was localized in cytoplasm, and Daxx immunoreactivity was translocated from nucleus to cytoplasm at 3 h after reperfusion. The TUNEL-positive cells could be found in peri-infarct region at 3 h and increased with time after reperfusion. Our findings suggest a possible association between expression of FADD, caspase-8, Daxx and FAP-1 genes and apoptosis following ischemia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
transient cerebral ischemia  ISOCasp8 (Rattus norvegicus)4142863; 4142863protein:increased expression:cerebral cortex:RGD 
transient cerebral ischemia  IEP 4142863protein:increased expression:cerebral cortex:RGD 
transient cerebral ischemia  ISODaxx (Rattus norvegicus)4142863; 4142863mRNA and protein:increased expression:cerebral cortex:RGD 
transient cerebral ischemia  IEP 4142863; 4142863; 4142863mRNA and protein:increased expression:cerebral cortex:RGD 
transient cerebral ischemia  ISOFadd (Rattus norvegicus)4142863; 4142863mRNA and protein:increased expression:cerebral cortex:RGD 
transient cerebral ischemia  ISOPtpn13 (Rattus norvegicus)4142863; 4142863mRNA and protein:increased expression:cerebral cortex:RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell body  IDA 4142863; 4142863; 4142863; 4142863large pyramidal neuronsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Casp8  (caspase 8)
Daxx  (death-domain associated protein)
Fadd  (Fas associated via death domain)
Ptpn13  (protein tyrosine phosphatase, non-receptor type 13)

Genes (Mus musculus)
Casp8  (caspase 8)
Daxx  (Fas death domain-associated protein)
Fadd  (Fas associated via death domain)
Ptpn13  (protein tyrosine phosphatase, non-receptor type 13)

Genes (Homo sapiens)
CASP8  (caspase 8)
DAXX  (death domain associated protein)
FADD  (Fas associated via death domain)
PTPN13  (protein tyrosine phosphatase non-receptor type 13)


Additional Information