RGD Reference Report - Cholecystokinin A and B receptors are differentially expressed in normal pancreas and pancreatic adenocarcinoma. - Rat Genome Database

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Cholecystokinin A and B receptors are differentially expressed in normal pancreas and pancreatic adenocarcinoma.

Authors: Weinberg, DS  Ruggeri, B  Barber, MT  Biswas, S  Miknyocki, S  Waldman, SA 
Citation: Weinberg DS, etal., J Clin Invest. 1997 Aug 1;100(3):597-603.
RGD ID: 4110818
Pubmed: PMID:9239407   (View Abstract at PubMed)
PMCID: PMC508227   (View Article at PubMed Central)
DOI: DOI:10.1172/JCI119570   (Journal Full-text)

Cholecystokinin (CCK) plays an important role in pancreatic carcinogenesis. While human CCK-A and -B receptors have been fully characterized, their relative roles in human pancreatic adenocarcinoma remain unclear. Thus, expression of CCK-A and -B receptors in normal human pancreas, pancreatic adenocarcinomas, and other human extrapancreatic tissues and malignancies was examined, using reverse transcription followed by the polymerase chain reaction (RT-PCR). mRNA isolated from 15 normal pancreas specimens, 22 pancreatic adenocarcinomas, and 58 extrapancreatic tissues and tumors was subjected to RT-PCR using primers specific for human CCK-A and -B receptors. Expression of CCK-B receptors was detected in all tissues arising from pancreas and in most extrapancreatic tissues and tumors. In contrast, CCK-A receptors exhibited a more selective pattern of expression in gall bladder, intestine, brain, ovary, spleen, and thymus. Of significance, CCK-A receptors were expressed selectively in all pancreatic adenocarcinomas, but not in any normal pancreas specimens. In situ hybridization, using receptor-specific riboprobes, localized CCK-A receptor expression to ductal cells, the presumed origin of most human pancreatic adenocarcinomas. Southern blot analysis revealed no evidence of CCK-A receptor gene amplification or rearrangement in pancreatic adenocarcinomas. Because of its selective expression, the CCK-A receptor may serve as selective biomarker for pancreatic adenocarcinoma.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
pancreatic ductal carcinoma  IEP 4110818mRNA:increased expression:pancreatic ductRGD 
pancreatic ductal carcinoma  ISOCCKAR (Homo sapiens)4110818; 4110818mRNA:increased expression:pancreatic ductRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cckar  (cholecystokinin A receptor)

Genes (Mus musculus)
Cckar  (cholecystokinin A receptor)

Genes (Homo sapiens)
CCKAR  (cholecystokinin A receptor)


Additional Information