RGD Reference Report - DIX domains of Dvl and axin are necessary for protein interactions and their ability to regulate beta-catenin stability. - Rat Genome Database

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DIX domains of Dvl and axin are necessary for protein interactions and their ability to regulate beta-catenin stability.

Authors: Kishida, S  Yamamoto, H  Hino, S  Ikeda, S  Kishida, M  Kikuchi, A 
Citation: Kishida S, etal., Mol Cell Biol. 1999 Jun;19(6):4414-22.
RGD ID: 4108508
Pubmed: PMID:10330181   (View Abstract at PubMed)
PMCID: PMC104400   (View Article at PubMed Central)

The N-terminal region of Dvl-1 (a mammalian Dishevelled homolog) shares 37% identity with the C-terminal region of Axin, and this related region is named the DIX domain. The functions of the DIX domains of Dvl-1 and Axin were investigated. By yeast two-hybrid screening, the DIX domain of Dvl-1 was found to interact with Dvl-3, a second mammalian Dishevelled relative. The DIX domains of Dvl-1 and Dvl-3 directly bound one another. Furthermore, Dvl-1 formed a homo-oligomer. Axin also formed a homo-oligomer, and its DIX domain was necessary. The N-terminal region of Dvl-1, including its DIX domain, bound to Axin directly. Dvl-1 inhibited Axin-promoted glycogen synthase kinase 3beta-dependent phosphorylation of beta-catenin, and the DIX domain of Dvl-1 was required for this inhibitory activity. Expression of Dvl-1 in L cells induced the nuclear accumulation of beta-catenin, and deletion of the DIX domain abolished this activity. Although expression of Axin in SW480 cells caused the degradation of beta-catenin and reduced the cell growth rate, expression of an Axin mutant that lacks the DIX domain did not affect the level of beta-catenin or the growth rate. These results indicate that the DIX domains of Dvl-1 and Axin are important for protein-protein interactions and that they are necessary for the ability of Dvl-1 and Axin to regulate the stability of beta-catenin.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Axin1  (axin 1)
Ctnnb1  (catenin beta 1)
Dvl3  (dishevelled segment polarity protein 3)


Additional Information