RGD Reference Report - A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function. - Rat Genome Database

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A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function.

Authors: Faulkner, NE  Dujardin, DL  Tai, CY  Vaughan, KT  O'Connell, CB  Wang, Y  Vallee, RB 
Citation: Faulkner NE, etal., Nat Cell Biol. 2000 Nov;2(11):784-91.
RGD ID: 4107032
Pubmed: PMID:11056532   (View Abstract at PubMed)
DOI: DOI:10.1038/35041020   (Journal Full-text)

Mutations in the LIS1 gene cause gross histological disorganization of the developing human brain, resulting in a brain surface that is almost smooth. Here we show that LIS1 protein co-immunoprecipitates with cytoplasmic dynein and dynactin, and localizes to the cell cortex and to mitotic kinetochores, which are known sites for binding of cytoplasmic dynein. Overexpression of LIS1 in cultured mammalian cells interferes with mitotic progression and leads to spindle misorientation. Injection of anti-LIS1 antibody interferes with attachment of chromosomes to the metaphase plate, and leads to chromosome loss. We conclude that LIS1 participates in a subset of dynein functions, and may regulate the division of neuronal progenitor cells in the developing brain.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of mitotic cell cycle  IMP 4107032 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pafah1b1  (platelet-activating factor acetylhydrolase 1b, regulatory subunit 1)


Additional Information