RGD Reference Report - Inhibition of 3beta- and 17beta-hydroxysteroid dehydrogenase activities in rat Leydig cells by perfluorooctane acid. - Rat Genome Database

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Inhibition of 3beta- and 17beta-hydroxysteroid dehydrogenase activities in rat Leydig cells by perfluorooctane acid.

Authors: Zhao, B  Chu, Y  Hardy, DO  Li, XK  Ge, RS 
Citation: Zhao B, etal., J Steroid Biochem Mol Biol. 2010 Jan;118(1-2):13-7. Epub 2009 Oct 7.
RGD ID: 2326078
Pubmed: PMID:19818404   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jsbmb.2009.09.010   (Journal Full-text)

Perfluorooctane acid (PFOA) is classified as a persistent organic pollutant and as an endocrine disruptor. The mechanism by which PFOA causes reduced testosterone production in males is not known. We tested our hypothesis that PFOA interferes with Leydig cell steroidogenic enzymes by measuring its effect on 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and 17beta-hydroxysteroid dehydrogenase 3 (17beta-HSD3) activities in rat testis microsomes and Leydig cells. The IC(50)s of PFOA and mode of inhibition were assayed. PFOA inhibited microsomal 3beta-HSD with an IC(50) of 53.2+/-25.9 microM and 17beta-HSD3 with an IC(50) 17.7+/-6.8 microM. PFOA inhibited intact Leydig cell 3beta-HSD with an IC(50) of 146.1+/-0.9 microM and 17beta-HSD3 with an IC(50) of 194.8+/-1.0 microM. The inhibitions of 3beta-HSD and 17beta-HSD3 by PFOA were competitive for the substrates. In conclusion, PFOA inhibits 3beta-HSD and 17beta-HSD3 in rat Leydig cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
steroid biosynthetic process  IDA 2326078 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
testosterone dehydrogenase (NAD+) activity  IDA 2326078 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hsd17b3  (hydroxysteroid (17-beta) dehydrogenase 3)


Additional Information