RGD Reference Report - In vivo formation of a proton-sensitive K+ channel by heteromeric subunit assembly of Kir5.1 with Kir4.1. - Rat Genome Database

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In vivo formation of a proton-sensitive K+ channel by heteromeric subunit assembly of Kir5.1 with Kir4.1.

Authors: Tanemoto, M  Kittaka, N  Inanobe, A  Kurachi, Y 
Citation: Tanemoto M, etal., J Physiol. 2000 Jun 15;525 Pt 3:587-92.
RGD ID: 2326048
Pubmed: PMID:10856114   (View Abstract at PubMed)
PMCID: PMC2269982   (View Article at PubMed Central)

Kir5.1 is an inwardly rectifying K+ channel (Kir) subunit, whose physiological function is unknown. Human embryonic kidney HEK293T cells co-transfected with rat Kir5.1 and Kir4.1 cDNA expressed a functional K+ channel, whose properties were significantly different from those of the homomeric Kir4.1 channel. Formation of a Kir4. 1/Kir5.1 assembly in HEK293T was confirmed biochemically. We found that heteromeric Kir4.1/Kir5.1 channel activity was affected by internal pH levels between 6.0 and 8.0, when the homomeric Kir4.1 channel activity was relatively stable. Changing external pH in this range had no effect on either Kir channel. Western blot analysis using specific antibodies revealed that Kir4.1 and Kir5.1 proteins were expressed in kidney and brain, but co-immunoprecipitated only from kidney. These results indicate that the co-assembly of Kir5.1 with Kir4.1 occurs in vivo, at least in kidney. The heteromeric Kir4. 1/Kir5.1 channel may therefore sense intracellular pH in renal epithelium and be involved in the regulation of acid-base homeostasis.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIKcnj16 (Rattus norvegicus)2326048 RGD 
protein binding  IPIKcnj10 (Rattus norvegicus)2326048 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnj10  (potassium inwardly-rectifying channel, subfamily J, member 10)
Kcnj16  (potassium inwardly-rectifying channel, subfamily J, member 16)


Additional Information