RGD Reference Report - Thrombus versus Wall Biological Activities in Experimental Aortic Aneurysms. - Rat Genome Database

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Thrombus versus Wall Biological Activities in Experimental Aortic Aneurysms.

Authors: Coutard, M  Touat, Z  Houard, X  Leclercq, A  Michel, JB 
Citation: Coutard M, etal., J Vasc Res. 2009 Dec 16;47(4):355-366.
RGD ID: 2325698
Pubmed: PMID:20016209   (View Abstract at PubMed)
DOI: DOI:10.1159/000265569   (Journal Full-text)

Background/Aim: The intraluminal thrombus (ILT) is considered to participate in abdominal aortic aneurysm (AAA) evolution. To assess whether this role proceeds via ILT influence on biological activity of the AAA wall, we studied the relationships between the levels of some relevant proteases and microparticles (MP) released by ILT versus wall in rat experimental AAAs. Methods and Results: Two weeks after elastase perfusion, ILT and AAA wall were incubated in cell culture medium and studies were performed on conditioned media. As shown by gelatin zymography, ILT released higher amounts of MMP9 than the wall, whereas the level of MMP2 activation (active/pro) was similar. Levels of elastase and urokinase plasminogen activator, plasmin and MPs, determined, respectively, by casein zymography, substrate hydrolysis and flow cytometry, were higher in ILT than in wall. Aneurysm diameter positively correlated with wall MMP9 levels, MMP2 activation, plasmin activity and MP release. Moreover, wall and ILT levels of pro- and active forms of MMP2, elastase and plasmin were positively correlated. Wall levels of MMP2 activation and plasmin activity also correlated with ILT weight. Conclusion: The present data suggest that, in this experimental model, ILT may contribute to AAA evolution via its influence on the level of aneurysmal wall protease activity.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
abdominal aortic aneurysm  ISOMmp2 (Rattus norvegicus)2325698; 2325698 RGD 
abdominal aortic aneurysm  IEP 2325698 RGD 
thrombosis  ISOPlau (Rattus norvegicus)2325698; 2325698protein:increased activity:extracellular region (rat)RGD 
thrombosis  IEP 2325698protein:increased activity:extracellular region (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mmp2  (matrix metallopeptidase 2)
Plau  (plasminogen activator, urokinase)

Genes (Mus musculus)
Mmp2  (matrix metallopeptidase 2)
Plau  (plasminogen activator, urokinase)

Genes (Homo sapiens)
MMP2  (matrix metallopeptidase 2)
PLAU  (plasminogen activator, urokinase)


Additional Information