RGD Reference Report - Methylation profile of circulating plasma DNA in patients with pancreatic cancer. - Rat Genome Database

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Methylation profile of circulating plasma DNA in patients with pancreatic cancer.

Authors: Melnikov, AA  Scholtens, D  Talamonti, MS  Bentrem, DJ  Levenson, VV 
Citation: Melnikov AA, etal., J Surg Oncol. 2009 Feb 1;99(2):119-22.
RGD ID: 2325022
Pubmed: PMID:19065635   (View Abstract at PubMed)
DOI: DOI:10.1002/jso.21208   (Journal Full-text)

BACKGROUND AND OBJECTIVES: Detection of pancreatic cancer by blood-based test may improve outcomes. We sought to establish the feasibility of a blood-based detection of pancreatic cancer through multiplexed array-mediated analysis of DNA methylation. METHODS: Methylation was assessed in each plasma sample using a panel of 56 frequently methylated genes. Methylation profiles in patients with ductal cell adenocarcinoma of the pancreas (n = 30) and healthy gender and age-matched controls (n = 30) were compared. Methylation was determined as described previously; a composite biomarker was developed for classification of cancer and normal samples. Sensitivity and specificity of the biomarker were estimated using 25 rounds of fivefold cross-validation. RESULTS: Five promoters were consistently selected for the classifier during cross-validation and comprised the final composite biomarker Five-fold cross-validation results indicate 76% sensitivity and 59% specificity of the biomarker, which included promoters of CCND2, SOCS1, THBS1, PLAU, and VHL. CONCLUSION: Differential methylation profiling of plasma DNA can detect ductal adenocarcinoma of the pancreas with significant accuracy and should be explored further. While additional improvement of biomarkers is necessary, the blood-based biomarker may be already useful as a first-line detection tool.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
pancreatic ductal carcinoma  IDA 2325022; 2325022DNA:hypermethylation:promoter (human)RGD 
pancreatic ductal carcinoma  ISOTHBS1 (Homo sapiens)2325022; 2325022DNA:hypermethylation:promoter (human)RGD 
pancreatic ductal carcinoma  ISOVHL (Homo sapiens)2325022; 2325022DNA:hypermethylation:promoter (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Thbs1  (thrombospondin 1)
Vhl  (von Hippel-Lindau tumor suppressor)

Genes (Mus musculus)
Thbs1  (thrombospondin 1)
Vhl  (von Hippel-Lindau tumor suppressor)

Genes (Homo sapiens)
THBS1  (thrombospondin 1)
VHL  (von Hippel-Lindau tumor suppressor)


Additional Information