RGD Reference Report - Characterization of stanniocalcin 2, a novel target of the mammalian unfolded protein response with cytoprotective properties. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Characterization of stanniocalcin 2, a novel target of the mammalian unfolded protein response with cytoprotective properties.

Authors: Ito, D  Walker, JR  Thompson, CS  Moroz, I  Lin, W  Veselits, ML  Hakim, AM  Fienberg, AA  Thinakaran, G 
Citation: Ito D, etal., Mol Cell Biol. 2004 Nov;24(21):9456-69.
RGD ID: 2324700
Pubmed: PMID:15485913   (View Abstract at PubMed)
PMCID: PMC522226   (View Article at PubMed Central)
DOI: DOI:10.1128/MCB.24.21.9456-9469.2004   (Journal Full-text)

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) induces a highly conserved homeostatic response in all eukaryotic cells, termed the unfolded-protein response (UPR). Here we describe the characterization of stanniocalcin 2 (STC2), a mammalian homologue of a calcium- and phosphate-regulating hormone first identified in fish, as a novel target of the UPR. Expression of STC2 gene is rapidly upregulated in cultured cells after exposure to tunicamycin and thapsigargin, by ATF4 after activation of the ER-resident kinase PERK. In addition, STC2 expression is also activated in neuronal cells by oxidative stress and hypoxia but not by several cellular stresses unrelated to the UPR. In contrast, expression of another homologue, STC1, is only upregulated by hypoxia independent of PERK or ATF4 expression. In vivo studies revealed that rat cortical neurons rapidly upregulate STC2 after transient middle cerebral artery occlusion. Finally, siRNA-mediated inhibition of STC2 expression renders N2a neuroblastoma cells and HeLa cells significantly more vulnerable to apoptotic cell death after treatment with thapsigargin, and overexpression of STC2 attenuated thapsigargin-induced cell death. Consequently, induced STC2 expression is an essential feature of survival component of the UPR.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Reperfusion Injury  ISOStc1 (Rattus norvegicus)2324700; 2324700mRNA:increased expression:cerebral cortexRGD 
Reperfusion Injury  ISOStc2 (Rattus norvegicus)2324700; 2324700mRNA and protein:increased expression:cerebral cortexRGD 
Reperfusion Injury  IEP 2324700mRNA:increased expression:cerebral cortexRGD 
Reperfusion Injury  IEP 2324700mRNA and protein:increased expression:cerebral cortexRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to hypoxia  IEP 2324700; 2324700 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Stc1  (stanniocalcin 1)
Stc2  (stanniocalcin 2)

Genes (Mus musculus)
Stc1  (stanniocalcin 1)
Stc2  (stanniocalcin 2)

Genes (Homo sapiens)
STC1  (stanniocalcin 1)
STC2  (stanniocalcin 2)


Additional Information