RGD Reference Report - The effect of old age on apolipoprotein E and its receptors in rat liver. - Rat Genome Database

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The effect of old age on apolipoprotein E and its receptors in rat liver.

Authors: Sabaretnam, T  O'Reilly, J  Kritharides, L  Le Couteur, DG 
Citation: Sabaretnam T, etal., Age (Dordr). 2009 Oct 7.
RGD ID: 2317724
Pubmed: PMID:19809892   (View Abstract at PubMed)
PMCID: PMC2829642   (View Article at PubMed Central)
DOI: DOI:10.1007/s11357-009-9115-2   (Journal Full-text)

Apolipoprotein E (apoE) is associated with aging and some age-related diseases. The majority of apoE is produced by hepatocytes for the receptor-mediated uptake of lipoproteins. Here, the effects of age on the hepatic expression and distribution of apoE and its receptors were determined using immunofluorescence, Western blots, and quantitative PCR in rat liver tissue and isolated hepatocytes. The expression of apoE mRNA and protein was not influenced significantly by aging. Immunofluorescence studies in isolated hepatocytes showed that apoE was more likely to be co-localized with early endosomes, golgi, and microtubules in isolated old hepatocytes. The mRNA expression of the receptor involved in sequestration of apoE, heparan sulfate proteoglycan was reduced in old age, without any significant effect on the expression of either the low-density lipoprotein receptor or low density-lipoprotein receptor-related protein. Old age is associated with changes in hepatic apoE intracellular trafficking and heparan sulfate proteoglycan expression that might contribute to age-related disease.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
early endosome located_inIDA 2317724 RGD 
microtubule located_inIDA 2317724 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apoe  (apolipoprotein E)


Additional Information