RGD Reference Report - Specific receptor subtype mediation of LPA-induced dual effects in cardiac fibroblasts. - Rat Genome Database

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Specific receptor subtype mediation of LPA-induced dual effects in cardiac fibroblasts.

Authors: Chen, J  Han, Y  Zhu, W  Ma, R  Han, B  Cong, X  Hu, S  Chen, X 
Citation: Chen J, etal., FEBS Lett. 2006 Aug 21;580(19):4737-45. Epub 2006 Jul 31.
RGD ID: 2317699
Pubmed: PMID:16890224   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2006.07.061   (Journal Full-text)

Lysophosphatidic acid (LPA) is a phospholipid messenger with diverse effects mediated via receptors LPA1, LPA2 and LPA3. Our previous study revealed that serum LPA level is elevated after myocardial infarction (MI). However, very little is known about the effects of LPA on cardiac fibroblasts (CFs) that play a crucial role in left ventricular remodeling after MI. Here we demonstrated that LPA dose-dependently induced proliferation and collagen synthesis with the maximum stimulation at 10 microM that was preferentially mediated by LPA3. LPA also dose-dependently induced apoptotic cell death, as estimated by MTT assay, hoechst staining, TUNEL and flow cytometric analysis, with an IC(50) of 50 microM. Moreover, apoptotic cell death may involve mitochondrial dysfunction and activation of caspase-3. Apoptosis induced by LPA might be mediated by LPA1. These data suggest that LPA exerts dual proliferative and proapoptotic actions mediated by specific LPA receptor subtypes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of apoptotic process  IMP 2317699 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lpar1  (lysophosphatidic acid receptor 1)


Additional Information