RGD Reference Report - HGF protects rat mesangial cells from high-glucose-mediated oxidative stress. - Rat Genome Database

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HGF protects rat mesangial cells from high-glucose-mediated oxidative stress.

Authors: Li, H  Jiang, T  Lin, Y  Zhao, Z  Zhang, N 
Citation: Li H, etal., Am J Nephrol. 2006;26(5):519-30. Epub 2006 Nov 22.
RGD ID: 2317504
Pubmed: PMID:17124385   (View Abstract at PubMed)
DOI: DOI:10.1159/000097368   (Journal Full-text)

BACKGROUND: Oxidative stress has been considered to be a common pathogenetic factor of diabetic nephropathy. Recent observations suggested that hepatocyte growth factor (HGF) was an antioxidant growth factor; thus, its renoprotective effects in diabetic nephropathy might be related to antioxidant mechanism. The aim of the present study was to evaluate whether HGF could prevent rat mesangial cells (RMC) from high-glucose-mediated oxidative stress and explore its relevant mechanism. METHODS: RMC were cultured in 5.6 mM (NG) or 30 mM (HG) glucose in the absence or presence of HGF (20 ng/ml) and c-met inhibitor SU11274 (5 microM) for 24 h. RESULTS: c-met expression in HG was markedly increased. Enhanced oxidative stress was observed in HG as evidenced by elevated reactive oxygen species and malondialdehyde levels and decreased glutathione level, which was markedly attenuated by HGF. HGF also inhibited HG-induced p22(phox) and aldose reductase upregulation and prevented HG-reduced glutamate-cysteine ligase catalytic subunit (GCLC) expression through inhibiting USF binding to negative regulatory region of GCLC promoter. Reduced glucose-6-phosphate dehydrogenase activity and expression in RMC by HG was rescued by HGF. CONCLUSION: HGF could function as an antioxidant factor and protect against HG-mediated oxidative stress by enhancing ROS scavenging and suppressing ROS production.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to glucose stimulus  IEP 2317504 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Met  (MET proto-oncogene, receptor tyrosine kinase)


Additional Information