RGD Reference Report - Cellular and subcellular localization of Na+-Ca2+ exchanger protein isoforms, NCX1, NCX2, and NCX3 in cerebral cortex and hippocampus of adult rat. - Rat Genome Database

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Cellular and subcellular localization of Na+-Ca2+ exchanger protein isoforms, NCX1, NCX2, and NCX3 in cerebral cortex and hippocampus of adult rat.

Authors: Minelli, A  Castaldo, P  Gobbi, P  Salucci, S  Magi, S  Amoroso, S 
Citation: Minelli A, etal., Cell Calcium. 2007 Mar;41(3):221-34. Epub 2006 Aug 17.
RGD ID: 2316976
Pubmed: PMID:16914199   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ceca.2006.06.004   (Journal Full-text)

Na(+)-Ca(2+) exchanger (NCX) controls cytosolic Ca(2+) and Na(+) concentrations ([Ca(2+)](i) and [Na(+)](i)) in eukaryotic cells. Here we investigated by immunocytochemistry the cellular and subcellular localization of the three known NCX isoforms, NCX1, NCX2 and NCX3, in adult rat neocortex and hippocampus. NCX1-3 were widely expressed in both brain areas: NCX1 immunoreactivity (ir) was exclusively associated to neuropilar puncta, while NCX2-3 were also detected in neuronal somata and dendrites. NCX1-3 ir was often identified around blood vessels. In both neocortex and hippocampus, all NCX isoforms were prominently expressed in dendrites and dendritic spines contacted by asymmetric axon terminals, whereas they were poorly expressed in presynaptic boutons. In addition, NCX1-3 ir was detected in astrocytes, notably in distal processes ensheathing excitatory synapses. All NCXs were expressed in perivascular astrocytic endfeet and endothelial cells. The robust expression of NCX1-3 in heterogeneous cell types in the brain in situ emphasizes their role in handling Ca(2+) and Na(+) in both excitable and non-excitable cells. Perisynaptic localization of NCX1-3 in dendrites and spines indicates that all isoforms are favourably located for buffering [Ca(2+)](i) in excitatory postsynaptic sites. NCX1-3 expressed in perisynaptic glial processes may participate in shaping astrocytic [Ca(2+)](i) transients evoked by ongoing synaptic activity.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell projection  IDA 2316976; 2316976; 2316976 RGD 
dendritic spine  IDA 2316976; 2316976; 2316976 RGD 
dendritic spine located_inIDA 2316976PMID:16914199SynGO 
microtubule  IDA 2316976; 2316976; 2316976 RGD 
neuronal cell body  IDA 2316976; 2316976 RGD 
postsynaptic membrane is_active_inIDA 2316976; 2316976; 2316976PMID:16914199SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Slc8a1  (solute carrier family 8 member A1)
Slc8a2  (solute carrier family 8 member A2)
Slc8a3  (solute carrier family 8 member A3)

Objects referenced in this article
Gene SLC8A3 solute carrier family 8 member A3 Homo sapiens

Additional Information