RGD Reference Report - Purification of 2,3-oxidosqualene cyclase from rat liver. - Rat Genome Database

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Purification of 2,3-oxidosqualene cyclase from rat liver.

Authors: Moore, WR  Schatzman, GL 
Citation: Moore WR and Schatzman GL, J Biol Chem. 1992 Nov 5;267(31):22003-6.
RGD ID: 2316919
Pubmed: PMID:1429550   (View Abstract at PubMed)

A rapid and simple purification of milligram amounts of 2,3-oxidosqualene cyclase, an integral membrane enzyme that catalyzes the cyclization of squalene epoxide to lanosterol, is reported. Several nonionic detergents (Triton X-100, Tween 80, Emulphogene, and lauryl maltoside) were evaluated for solubilization of oxidosqualene cyclase from rat liver microsomes. At a detergent concentration of 5 mg/ml, lauryl maltoside was approximately 10 times more effective than Emulphogene in the solubilization of oxidosqualene cyclase; Triton X-100 and Tween 80 were less effective than Emulphogene as judged by the relative specific activities of the solubilized enzyme. Treatment of microsomes with lauryl maltoside resulted in a selective solubilization of the cyclase with concomitant activation of the enzyme. The solubilized enzyme was purified to homogeneity by fast protein liquid chromatography. The purified enzyme consists of a single subunit that has an apparent molecular weight of 65,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme obeys saturation kinetics and the apparent Km of (2,3)-oxidosqualene is 15 microM; the apparent kcat/Km is 200 M-1.min-1. An improved assay of the enzyme that utilizes high performance liquid chromatography methods is also described.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cholesterol biosynthetic process  IDA 2316919 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lanosterol synthase activity  IDA 2316919 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cholesterol biosynthetic pathway   ISOLss (Rattus norvegicus)2316919; 2316919 RGD 
cholesterol biosynthetic pathway   IDA 2316919 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Lss  (lanosterol synthase)

Genes (Mus musculus)
Lss  (lanosterol synthase)

Genes (Homo sapiens)
LSS  (lanosterol synthase)


Additional Information