RGD Reference Report - Overexpression of AMPKalpha1 Ameliorates Fatty Liver in Hyperlipidemic Diabetic Rats. - Rat Genome Database

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Overexpression of AMPKalpha1 Ameliorates Fatty Liver in Hyperlipidemic Diabetic Rats.

Authors: Seo, E  Park, EJ  Joe, Y  Kang, S  Kim, MS  Hong, SH  Park, MK  Kim, DK  Koh, H  Lee, HJ 
Citation: Seo E, etal., Korean J Physiol Pharmacol. 2009 Dec;13(6):449-54. Epub 2009 Dec 31.
RGD ID: 2316808
Pubmed: PMID:20054491   (View Abstract at PubMed)
PMCID: PMC2802305   (View Article at PubMed Central)
DOI: DOI:10.4196/kjpp.2009.13.6.449   (Journal Full-text)

5'-AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic (alpha) and two regulatory (beta and gamma) subunits. Two isoforms are known for catalytic subunit (alpha1, alpha2) and are encoded by different genes. To assess the metabolic effects of AMPKalpha1, we examined the effects of overexpression of adenoviral-mediated AMPKalpha1 in hyperlipidemic type 2 diabetic rats. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is an established animal model of type 2 diabetes that exhibits chronic and slowly progressive hyperglycemia and hyperlipidemia. Thirty five-week-old overt type 2 diabetic rats (n=10) were administered intravenously with Ad.AMPKalpha1. AMPK activity was measured by phosphorylation of acetyl CoA carboxlyase (ACC). To investigate the changes of gene expression related glucose and lipid metabolism, quantitative real-time PCR was performed with liver tissues. Overexpression of AMPKalpha1 showed that blood glucose concentration was decreased but that glucose tolerance was not completely recovered on 7th day after treatment. Plasma triglyceride concentration was decreased slightly, and hepatic triglyceride content was markedly reduced by decreasing expression of hepatic lipogenic genes. Overexpression of AMPKalpha1 markedly improved hepatic steatosis and it may have effective role for improving hepatic lipid metabolism in hyperlipidemic state.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
type 2 diabetes mellitus  ISOPrkaa1 (Rattus norvegicus)2316808; 2316808 RGD 
type 2 diabetes mellitus  IMP 2316808 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prkaa1  (protein kinase AMP-activated catalytic subunit alpha 1)

Genes (Mus musculus)
Prkaa1  (protein kinase, AMP-activated, alpha 1 catalytic subunit)

Genes (Homo sapiens)
PRKAA1  (protein kinase AMP-activated catalytic subunit alpha 1)


Additional Information