RGD Reference Report - Ultrastructural localization of delta-1 opioid receptor in the dorsal raphe nucleus of the rat. - Rat Genome Database

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Ultrastructural localization of delta-1 opioid receptor in the dorsal raphe nucleus of the rat.

Authors: Wang, QP  Ochiai, H  Guan, JL  Nakai, Y 
Citation: Wang QP, etal., Synapse. 1997 Jul;26(3):243-53.
RGD ID: 2316596
Pubmed: PMID:9183814   (View Abstract at PubMed)
DOI: DOI:10.1002/(SICI)1098-2396(199707)26:3<243::AID-SYN6>3.0.CO;2-7   (Journal Full-text)

The ultrastructural localization of delta-1 opioid receptor in the rat dorsal raphe nucleus was studied by the preembedding avidin-biotin-peroxidase complex technique. With application of a low concentration of the first antiserum in incubation and control of short-time reaction to 3,3'-diaminobenzidine, the immunoreaction seemed to be faint at the light microscopic level. At the electron microscopic level, however, delta-1 opioid receptor immunoreaction products were found to be localized specifically on the postsynaptic membrane of dendrites, dense-cored vesicles, and the surface of the small, clear vesicles in axon terminals with strong immunoreactivity. Of the total 659 immunopositive profiles observed, up to 62.4% (411/659) were dendrites, whereas 33.8% (223/659) were axon terminals. The immunostained myelinated axons and perikarya were relatively rare, with the frequencies 0.8% (5/659) and 3.0% (20/659), respectively. Most of the immunopositive dendrites (338/411, 82.2%) were immunostained only at the postsynaptic membranes. Other immunoreactive dendrites showed their immunoreaction products also in some other contents besides the postsynaptic membranes (44/411, 10.7%) or only in those contents but not the postsynaptic membranes (25/411, 6.1%). Only four dendrites showed their immunoreactive results only at the membrane not related to synapse (4/267, 1.0%). No dendrite was found immunostained in all the contents. About half of the immunopositive axon terminals (125/223, 56.1%) were found to make synapse with nonimmunoreactive dendrites (76/223, 34.1%) or immunoreactive dendrites (49/223, 22.0%), while only one was found to make contact with immunoreactive perikarya. The present study showed that delta-1 opioid receptor in the dorsal raphe nucleus is mostly localized on postsynaptic membrane; the main function of the delta-1 receptor in the dorsal raphe nucleus is to receive signals from the opioid-containing axon terminals through synapses.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axon terminus  IDA 2316596 RGD 
dendrite membrane  IDA 2316596 RGD 
postsynaptic membrane  IDA 2316596 RGD 
vesicle  IDA 2316596 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Oprd1  (opioid receptor, delta 1)


Additional Information