RGD Reference Report - Overexpression of cytosolic sialidase Neu2 induces myoblast differentiation in C2C12 cells. - Rat Genome Database

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Overexpression of cytosolic sialidase Neu2 induces myoblast differentiation in C2C12 cells.

Authors: Fanzani, A  Giuliani, R  Colombo, F  Zizioli, D  Presta, M  Preti, A  Marchesini, S 
Citation: Fanzani A, etal., FEBS Lett. 2003 Jul 17;547(1-3):183-8.
RGD ID: 2316560
Pubmed: PMID:12860410   (View Abstract at PubMed)

Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To understand the biological significance of Neu2 upregulation in myoblast differentiation, C2C12 cells were stably transfected with the rat cytosolic sialidase Neu2 cDNA. Neu2 overexpressing clones were characterized by a marked decrement of cell proliferation and by the capacity to undergo spontaneous myoblast differentiation also when maintained under standard growth conditions. This was evidenced by the formation of myogenin-positive myotubes and by a significant decrease in the nuclear levels of cyclin D1 protein. No differentiation was on the contrary observed in parental and mock-transfected cells under the same experimental conditions. The results indicate that Neu2 upregulation per se is sufficient to trigger myoblast differentiation in C2C12 cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of myoblast differentiation  IDA 2316560 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Neu2  (neuraminidase 2)


Additional Information