RGD Reference Report - An in-vivo analysis of capillary stasis and endothelial apoptosis in a model of hypertension. - Rat Genome Database

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An in-vivo analysis of capillary stasis and endothelial apoptosis in a model of hypertension.

Authors: Tran, ED  Schmid-Schonbein, GW 
Citation: Tran ED and Schmid-Schonbein GW, Microcirculation. 2007 Nov-Dec;14(8):793-804.
RGD ID: 2316357
Pubmed: PMID:17924279   (View Abstract at PubMed)
DOI: DOI:10.1080/10739680701419992   (Journal Full-text)

OBJECTIVE: Recent evidence suggests endothelial apoptosis may be a mechanism for capillary rarefaction in hypertensives. The objective of this study was to examine the early phase of endothelial apoptosis and capillary blood flow in the spontaneously hypertensive rat (SHR) and the normotensive Wistar-Kyoto (WKY) rat. METHODS: Since hypertension in SHR is dependent on glucocorticoids, the animals were treated with dexamethasone (DEX), by intraperitoneal injection and then by superfusion on exposed mesentery. Selected capillaries were continuously observed. Annexin V and propidium iodide were used to detect apoptosis. RESULTS: Without central pressure reduction, permanent capillary stasis was initiated by the entrapment of leukocytes at the location of an endothelial cell that had platelets attached to it. Apoptosis of the endothelial cell was followed by apoptosis in other endothelial cells of the obstructed capillary. The incidence of stasis and total cell death in WKY+DEX were higher than WKY, whereas there were no differences between SHR+DEX and SHR. Blockade of the lectin do main of L-selectin or a platelet membrane adhesion molecule (glycoprotein IIb/IIIa) blocked the development of stasis. CONCLUSIONS: Glucocorticoid facilitates cell death and microvessel stasis. Immobilized platelets and leukocytes play a central role in capillary stasis, which leads to progression of endothelial apoptosis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
peripheral vascular disease  ISOSell (Rattus norvegicus)2316357; 2316357 RGD 
peripheral vascular disease  IMP 2316357 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Sell  (selectin L)

Genes (Mus musculus)
Sell  (selectin, lymphocyte)

Genes (Homo sapiens)
SELL  (selectin L)

Objects referenced in this article
Gene Itga2b integrin subunit alpha 2b Rattus norvegicus
Gene Itgb3 integrin subunit beta 3 Rattus norvegicus

Additional Information