RGD Reference Report - Growth retardation in rats whose growth hormone gene expression was suppressed by antisense RNA transgene. - Rat Genome Database

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Growth retardation in rats whose growth hormone gene expression was suppressed by antisense RNA transgene.

Authors: Matsumoto, K  Kakidani, H  Takahashi, A  Nakagata, N  Anzai, M  Matsuzaki, Y  Takahashi, Y  Miyata, K  Utsumi, K  Iritani, A 
Citation: Matsumoto K, etal., Mol Reprod Dev. 1993 Sep;36(1):53-8.
RGD ID: 2315643
Pubmed: PMID:8398130   (View Abstract at PubMed)
DOI: DOI:10.1002/mrd.1080360109   (Journal Full-text)

We produced four transgenic founder rats (F0) by introducing into rat embryos a fusion gene, which consisted of rat growth hormone (GH) promoter containing with four copies of thyroid hormone response element (TRE) and antisense cDNA sequences for rat GH. This transgene promoter directed 2.8-fold stimulation of CAT gene expression in transfected GH3 rat pituitary tumor cells compared with the rat GH promoter alone. Two of four transgenic rats expressed antisense RNA in the pituitary. Transgenic offspring (F1) from each founder rat exhibited dwarfism at as early as 3-4 weeks of age, and they exhibited approximately 70-85% reduced growth rate compared with their nontransgenic littermates over 56 weeks of observation. Plasma rat GH concentration was approximately 40-50% lower in transgenic F1 rats compared to their nontransgenic littermates. In these experiments, the pituitary hormone expression controlled in a complex manner was shown to be repressed by the antisense RNA transgene. Furthermore, the suppression of gene expression could be achieved by antisense RNA transgene in the rat as well.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of growth  IMP 2315643 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gh1  (growth hormone 1)

Objects referenced in this article
Strain W-Tg(Gh1as)Nibs null Rattus norvegicus

Additional Information