RGD Reference Report - Zhonghua wai ke za zhi [Chinese journal of surgery] - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Zhonghua wai ke za zhi [Chinese journal of surgery]

Authors: Zhao, D  Yang, BX 
Citation: Zhao D and Yang BX, Zhonghua Wai Ke Za Zhi. 2009 Mar 1;47(5):373-6.
RGD ID: 2314952
Pubmed: PMID:19595018   (View Abstract at PubMed)

OBJECTIVE: To examine the early changes in gene expression levels in lung tissues by cDNA microarray using a rat model of total hepatic ischemia reperfusion and to analysis function of the changes. METHODS: Twelve adult male SD rats weighting 220 - 250 g were divided randomly into two groups (6 in each group). The rats were sacrificed at end point of the operation and lung tissues were divided into several parts for either microarray analysis or RT-PCR of several genes selected from microarray data. Common change genes were selected from three chips and the final results of microarray analysis were identified by RT-PCR. At last, differentially expressed genes were classified according to their biological functions by cluster analysis. RESULTS: Analysis of the results showed those 48 genes up-regulated and 32 genes down-regulated after hepatic ischemia reperfusion lung injury. Only parts of them had we known about the function. Genes significantly up-regulated were IL-1 alpha, IL-1 beta, SLPI, MMP9, MMP14, MMP15, TIMP1, PIK3RL, MAPK, NF-kappaB, JNK and others. Genes significantly down-regulated were CYP1A1, NQO1, GSTA3, RETNLA and others. Differentially expressed genes were mainly classified into inflammatory reaction, transcription factors, cell metabolism, signals transduction, ion or receptors, cytoskeleton, etc. CONCLUSIONS: cDNA microarray technique provides a new method for detecting differentially expressed genes in rat lung tissues. Further study may reveal the molecular pathologic mechanism of hepatic ischemia reperfusion lung injury and discern new targets for therapeutic interventions.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Liver Reperfusion Injury  ISOCyp1a1 (Rattus norvegicus)2314952; 2314952mRNA:decreased expression:lungRGD 
Liver Reperfusion Injury  IEP 2314952; 2314952mRNA:decreased expression:lungRGD 
Liver Reperfusion Injury  ISOGstm1 (Rattus norvegicus)2314952; 2314952mRNA:decreased expression:lungRGD 
Liver Reperfusion Injury  ISOIl1a (Rattus norvegicus)2314952; 2314952mRNA:increased expression:lungRGD 
Liver Reperfusion Injury  IEP 2314952; 2314952mRNA:increased expression:lungRGD 
Liver Reperfusion Injury  ISOMmp15 (Rattus norvegicus)2314952; 2314952mRNA:increased expression:lungRGD 
Liver Reperfusion Injury  ISOSlpi (Rattus norvegicus)2314952; 2314952 RGD 
Liver Reperfusion Injury  IEP 2314952 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp1a1  (cytochrome P450, family 1, subfamily a, polypeptide 1)
Gstm1  (glutathione S-transferase mu 1)
Il1a  (interleukin 1 alpha)
Mmp15  (matrix metallopeptidase 15)
Slpi  (secretory leukocyte peptidase inhibitor)

Genes (Mus musculus)
Cyp1a1  (cytochrome P450, family 1, subfamily a, polypeptide 1)
Gstm1  (glutathione S-transferase, mu 1)
Il1a  (interleukin 1 alpha)
Mmp15  (matrix metallopeptidase 15)
Slpi  (secretory leukocyte peptidase inhibitor)

Genes (Homo sapiens)
CYP1A1  (cytochrome P450 family 1 subfamily A member 1)
GSTM1  (glutathione S-transferase mu 1)
IL1A  (interleukin 1 alpha)
MMP15  (matrix metallopeptidase 15)
SLPI  (secretory leukocyte peptidase inhibitor)


Additional Information