RGD Reference Report - Neutralization of the membrane protein Nogo-A enhances growth and reactive sprouting in established organotypic hippocampal slice cultures. - Rat Genome Database

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Neutralization of the membrane protein Nogo-A enhances growth and reactive sprouting in established organotypic hippocampal slice cultures.

Authors: Craveiro, LM  Hakkoum, D  Weinmann, O  Montani, L  Stoppini, L  Schwab, ME 
Citation: Craveiro LM, etal., Eur J Neurosci. 2008 Nov;28(9):1808-24.
RGD ID: 2314943
Pubmed: PMID:18973596   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1460-9568.2008.06473.x   (Journal Full-text)

The reduced ability of central axons to regenerate after injury is significantly influenced by the presence of several molecules that inhibit axonal growth. Nogo-A is one of the most studied and most potent of the myelin-associated growth inhibitory molecules. Its neutralization, as well as interference with its signalling, allows for enhanced axonal sprouting and growth following injury. Using differentiated rat organotypic hippocampal slice cultures treated for 5 days with either of two different function-blocking anti-Nogo-A antibodies, we show an increase in CA3 fibre regeneration after lesion. In intact slices, 5 days of anti-Nogo-A antibody treatment led to increased sprouting of intact CA3 fibres that are positive for neurofilament 68. A transcriptomic approach confirmed the occurrence of a growth response on the molecular level upon Nogo-A neutralization in intact cultures. Our results demonstrate that Nogo-A neutralization for 5 days is sufficient for the induction of growth in mature CNS tissue without the prerequisite of an injury. Nogo-A may therefore act as a tonic growth suppressor/stabilizer in the adult intact hippocampus.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of axonogenesis  IMP 2314943 RGD 
negative regulation of neuron projection regeneration  IMP 2314943 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rtn4  (reticulon 4)


Additional Information