RGD Reference Report - The Rab27 effector Rabphilin, unlike Granuphilin and Noc2, rapidly exchanges between secretory granules and cytosol in PC12 cells. - Rat Genome Database

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The Rab27 effector Rabphilin, unlike Granuphilin and Noc2, rapidly exchanges between secretory granules and cytosol in PC12 cells.

Authors: Handley, MT  Burgoyne, RD 
Citation: Handley MT and Burgoyne RD, Biochem Biophys Res Commun. 2008 Aug 22;373(2):275-81. Epub 2008 Jun 23.
RGD ID: 2314875
Pubmed: PMID:18573236   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2008.06.043   (Journal Full-text)

Rab proteins are GTPases that transit between GTP- and GDP-bound states. In the GTP-bound form they can recruit specific effector to membrane domains. It is possible that the exchange of Rab effectors between membranes and cytosol would be determined by the exchange of the particular Rab partner. We have compared the cycling of three Rab3/27 effectors, Granuphilin, Noc2, and Rabphilin, in PC12 cells using fluorescence recovery after photobleaching of EGFP-tagged proteins. All three effectors become localised to secretory granules. Granuphilin and Noc2 showed little or no exchange between secretory granules and cytosol whereas Rabphilin showed rapid and complete exchange. Both Noc2 and Rabphilin were found to be recruited to granules by Rab27 but the data suggest that Rabphilin did not form stable complexes with Rab27 on secretory granules and so Rab effector cycling between membranes and cytosol can be independent of that of the Rab protein.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
secretory granule  IDA 2314875 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rph3a  (rabphilin 3A)


Additional Information