RGD Reference Report - Characteristics of Cl- uptake in rat alveolar type I cells. - Rat Genome Database

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Characteristics of Cl- uptake in rat alveolar type I cells.

Authors: Johnson, M  Allen, L  Dobbs, L 
Citation: Johnson M, etal., Am J Physiol Lung Cell Mol Physiol. 2009 Nov;297(5):L816-27. Epub 2009 Aug 14.
RGD ID: 2314617
Pubmed: PMID:19684200   (View Abstract at PubMed)
PMCID: PMC2777494   (View Article at PubMed Central)
DOI: DOI:10.1152/ajplung.90466.2008   (Journal Full-text)

Although Cl- transport in fetal lung is important for fluid secretion and normal lung development, the role of Cl- transport in adult lung is not well understood. In physiological studies, the cystic fibrosis transmembrane regulator (CFTR) plays a role in fluid absorption in the distal air spaces of adult lung, and alveolar type II cells cultured for 5 days have the capacity to transport Cl-. Although both alveolar type I and type II cells express CFTR, it has previously not been known whether type I cells transport Cl-. We studied Cl- uptake in isolated type I cells directly, using either radioisotopic tracers or halide-sensitive fluorescent indicators. By both methods, type I cells take up Cl-. In the presence of beta-adrenergic agonist stimulation, Cl- uptake can be inhibited by CFTR antagonists. Type I cells express both the Cl-/HCO3- anion exchanger AE2 and the voltage-gated Cl- channels CLC5 and CLC2. Inhibitors of AE2 also block Cl- uptake in type I cells. Together, these results demonstrate that type I cells are capable of Cl- uptake and suggest that the effects seen in whole lung studies establishing the importance of Cl- movement in alveolar fluid clearance may be, in part, the result of Cl- transport across type I cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
chloride transport  IMP 2314617 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cftr  (CF transmembrane conductance regulator)


Additional Information