RGD Reference Report - NAD(P)H oxidase, superoxide dismutase, catalase, glutathione peroxidase and nitric oxide synthase expression in subacute spinal cord injury. - Rat Genome Database

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NAD(P)H oxidase, superoxide dismutase, catalase, glutathione peroxidase and nitric oxide synthase expression in subacute spinal cord injury.

Authors: Vaziri, ND  Lee, YS  Lin, CY  Lin, VW  Sindhu, RK 
Citation: Vaziri ND, etal., Brain Res. 2004 Jan 2;995(1):76-83.
RGD ID: 2314450
Pubmed: PMID:14644473   (View Abstract at PubMed)

Primary trauma to the spinal cord triggers a cascade of cellular and molecular events that promote continued tissue damage and expansion of the lesion for extended periods following the initial injury. Oxidative and nitrosative stresses play an important role in progression of spinal cord injury (SCI). In an attempt to explore the biochemical origin of oxidative/nitrosative stress associated with secondary SCI, we studied expression of the superoxide (O2*-)-generating enzyme, NAD(P)H oxidase, antioxidant enzymes [superoxide dismutase (CuZn SOD, Mn SOD), catalase, glutathione peroxidase (GPX)], nitric oxide synthases (NOS) and a byproduct of NO-O2*- interaction (nitrotyrosine) in the spinal cord tissues of rats 16 h and 14 days after surgical resections of a 5-mm segment of the cord below T8 or sham-operation. Immunodetectable NAD(P)H oxidase subunits (gp91phox and P67phox), Mn SOD, inducible NOS (iNOS), endothelial NOS (eNOS), and nitrotyrosine were elevated in the transected cords on day 1 and day 14. Neuronal NOS (nNOS) was unchanged on day 1 and significantly depressed on day 14. GPX was unchanged on day 1 and significantly elevated on day 14. Catalase was unchanged in the cord tissue surrounding the transection site at both points. Thus, concurrent upregulations of NAD(P)H oxidase, eNOS and iNOS (but not nNOS), work in concert to maintain oxidative and nitrosative stress in the injured cord tissue.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Spinal Cord Injuries  ISONcf2 (Rattus norvegicus)2314450; 2314450protein:increased expression:spinal cordRGD 
Spinal Cord Injuries  IEP 2314450protein:increased expression:spinal cordRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncf2  (neutrophil cytosolic factor 2)

Genes (Mus musculus)
Ncf2  (neutrophil cytosolic factor 2)

Genes (Homo sapiens)
NCF2  (neutrophil cytosolic factor 2)


Additional Information