RGD Reference Report - Severe food restriction induces myocardial dysfunction related to SERCA2 activity. - Rat Genome Database

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Severe food restriction induces myocardial dysfunction related to SERCA2 activity.

Authors: Sugizaki, MM  Leopoldo, AS  Okoshi, MP  Bruno, A  Conde, SJ  Lima-Leopoldo, AP  Padovani, CR  Carvalho, RF  Do Nascimento, AF  De Campos, DH  Nogueira, CR  Cicogna, AC 
Citation: Sugizaki MM, etal., Can J Physiol Pharmacol. 2009 Sep;87(9):666-73.
RGD ID: 2314312
Pubmed: PMID:19794517   (View Abstract at PubMed)
DOI: DOI:10.1139/y09-060   (Journal Full-text)

Previous studies have shown that food restriction promotes myocardial dysfunction in rats. However, the molecular mechanisms that are responsible are unclear. We investigated the role of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) on myocardial performance in food-restricted rats. Male Wistar-Kyoto rats, 60 days old, were fed a control or restricted diet (daily energy intake reduced to 50% of the control) for 90 days. Expression of Serca2a, phospholamban (PLB), Na+/Ca2+ exchanger (NCX), and thyroid hormone receptor (TRalpha1, TRbeta1) mRNA was determined by quantitative PCR. SERCA2 activity was measured by using 20 micromol/L cyclopiazonic acid (CPA) in a left ventricular papillary muscle preparation during isometric contraction in basal conditions and during post-rest contraction. Serum concentrations of thyroxine (T4) and thyrotropin (TSH) were also determined. The 50%-restricted diet reduced body and ventricular weight and serum T4 and TSH levels. The interaction of CPA and food restriction reduced peak developed tension and maximum rate of tension decline (-dT/dt), but increased the resting tension intensity response during post-rest contraction. PLB and NCX mRNA were upregulated and TRalpha1 mRNA was downregulated by food restriction. These results suggest that food restriction promotes myocardial dysfunction related to impairment of sarcoplasmic reticulum Ca2+ uptake as a result of a hypothyroid state.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to nutrient levels  IEP 2314312 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Thra  (thyroid hormone receptor alpha)


Additional Information