RGD Reference Report - A p300 protein as a coactivator of GATA-6 in the transcription of the smooth muscle-myosin heavy chain gene. - Rat Genome Database

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A p300 protein as a coactivator of GATA-6 in the transcription of the smooth muscle-myosin heavy chain gene.

Authors: Wada, H  Hasegawa, K  Morimoto, T  Kakita, T  Yanazume, T  Sasayama, S 
Citation: Wada H, etal., J Biol Chem. 2000 Aug 18;275(33):25330-5.
RGD ID: 2314198
Pubmed: PMID:10851229   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M000828200   (Journal Full-text)

The mechanisms that regulate smooth muscle development and differentiation are poorly understood. Although recent studies have suggested the possible role of a zinc finger transcription factor, GATA-6, in the differentiation of vascular smooth muscle cells (VSMCs), the downstream gene targeted by GATA-6 is unknown. The expression of smooth muscle-myosin heavy chain (Sm-MHC) provides a highly specific marker for the differentiated phenotype of VSMCs as well as the smooth muscle cell lineage. Here, we show that GATA-6 bound to a GATA-like motif (-810/-805) within the rat Sm-MHC promoter in a sequence-specific manner and activated this promoter through this site. In addition, we show that the transcriptional coactivator p300 associated with GATA-6 during the transcription of the Sm-MHC gene. A p300/GATA-6 complex in VSMCs was up-regulated by induction of the quiescent phenotype. A wild-type E1A, which interferes with endogenous p300, but not a mutant E1A defective for p300 binding, markedly down-regulated the expression of endogenous Sm-MHC in quiescent-phenotype VSMCs. These studies provide the first identification of a functionally important GATA-6 binding site within a smooth muscle-specific promoter and suggest a role for p300 in the maintenance of the differentiated phenotype in VSMCs as a coactivator of GATA-6.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
double-stranded DNA binding  IDA 2314198 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gata6  (GATA binding protein 6)


Additional Information