RGD Reference Report - Decrease of peroxisome proliferator-activated receptor delta expression in cardiomyopathy of streptozotocin-induced diabetic rats. - Rat Genome Database

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Decrease of peroxisome proliferator-activated receptor delta expression in cardiomyopathy of streptozotocin-induced diabetic rats.

Authors: Yu, BC  Chang, CK  Ou, HY  Cheng, KC  Cheng, JT 
Citation: Yu BC, etal., Cardiovasc Res. 2008 Oct 1;80(1):78-87. Epub 2008 Jun 23.
RGD ID: 2313781
Pubmed: PMID:18573863   (View Abstract at PubMed)
DOI: DOI:10.1093/cvr/cvn172   (Journal Full-text)

AIMS: The role of peroxisome proliferator-activated receptor delta (PPARdelta) in the development of cardiomyopathy, which is widely observed in diabetic disorders, is likely because cardiomyocyte-restricted PPARdelta deletion causes cardiac hypertrophy. Thus, we investigated the effect of hyperglycaemia-induced oxidative stress on the expression of cardiac PPARdelta both in vivo and in vitro. METHODS AND RESULTS: We used male Wistar rats to examine the effect of hyperglycaemia on PPARdelta expression in streptozotocin-induced diabetic rats, primary neonatal rat cardiomyocytes, and H9c2 embryonic rat cardiomyocytes. PPARdelta mRNA (messenger ribonucleic acid) and protein levels were measured using northern and western blotting, respectively. The lipid deposition within the heart section was assessed by oil red O staining. The formation of reactive oxygen species (ROS) and changes in morphology, protein synthesis, and alpha-actinin content in hyperglycaemic cells were also examined. Inhibitors of ROS production or mitogen-activated protein kinase (MAPK) activation were employed to investigate the possible mechanisms. Cardiomyopathy induced in streptozotocin-diabetic rats was associated with a marked decrease in cardiac PPARdelta expression. Also, ROS production, cell size, and protein synthesis were increased while PPARdelta expression was reduced in cells exposed to hyperglycaemia in vitro. However, these glucose-induced changes were abolished in the presence of tiron or PD98059 (MEK/ERK inhibitor). CONCLUSION: Our results suggest that inhibitors of ROS production or MAPK activation are involved in reduction of cardiac PPARdelta expression in response to hyperglycaemia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Diabetes Mellitus  ISOPpard (Rattus norvegicus)2313781; 2313781mRNA and protein:decreased expression:heartRGD 
Experimental Diabetes Mellitus  IEP 2313781mRNA and protein:decreased expression:heartRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to glucose  IEP 2313781 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ppard  (peroxisome proliferator-activated receptor delta)

Genes (Mus musculus)
Ppard  (peroxisome proliferator activator receptor delta)

Genes (Homo sapiens)
PPARD  (peroxisome proliferator activated receptor delta)


Additional Information