RGD Reference Report - Erythropoietin and bone morphogenetic protein 7 mediate ascorbate-induced dopaminergic differentiation from embryonic mesencephalic precursors. - Rat Genome Database

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Erythropoietin and bone morphogenetic protein 7 mediate ascorbate-induced dopaminergic differentiation from embryonic mesencephalic precursors.

Authors: Lee, JY  Koh, HC  Chang, MY  Park, CH  Lee, YS  Lee, SH 
Citation: Lee JY, etal., Neuroreport. 2003 Jul 18;14(10):1401-4.
RGD ID: 2313656
Pubmed: PMID:12876482   (View Abstract at PubMed)
DOI: DOI:10.1097/01.wnr.0000078542.07662.4b   (Journal Full-text)

Mesencephalic precursors derived from early (embryonic day 12; E12) rat embryos were grown in vitro using mitogen basic fibroblast growth factor (bFGF) and these cells efficiently differentiated into dopaminergic (DA) neurons. However, this in vitro DA differentiation was poor in mesencephalic precursors isolated from later embryos (E13-15). Ascorbate (AA) treatment enhanced yields of DA neurons from E12 precursors, and increased the number of DA neurons generated from E13 precursors to levels attained when using E12 precursors. AA markedly up-regulated expression of bone morphogenetic protein 7 (BMP7) and erythropoietin (Epo) in precursors, but did not affect expression of a number of genes known to regulate midbrain DA development. The addition of these recombinant proteins or blockers revealed that both BMP7 and Epo mediate AA-induced DA neuron differentiation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of neuron differentiation  IDA 2313656 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Epo  (erythropoietin)


Additional Information