RGD Reference Report - Protein phosphatase 2A is the main phosphatase involved in the regulation of protein kinase B in rat adipocytes. - Rat Genome Database

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Protein phosphatase 2A is the main phosphatase involved in the regulation of protein kinase B in rat adipocytes.

Authors: Resjo, S  Goransson, O  Harndahl, L  Zolnierowicz, S  Manganiello, V  Degerman, E 
Citation: Resjo S, etal., Cell Signal. 2002 Mar;14(3):231-8.
RGD ID: 2313392
Pubmed: PMID:11812651   (View Abstract at PubMed)

In adipocytes, protein kinase B (PKB) has been suggested to be the enzyme that phosphorylates phosphodiesterase 3B (PDE3B), a key enzyme in insulin's antilipolytic signalling pathway. In order to screen for PKB phosphatases, adipocyte homogenates were fractionated using ion-exchange chromatography and analysed for PKB phosphatase activities. PKB phosphatase activity eluted as one main peak, which coeluted with serine/threonine phosphatases (PP)2A. In addition, adipocytes were incubated with inhibitors of PP. Incubation of adipocytes with 1 microM okadaic acid inhibited PP2A by 75% and PP1 activity by only 17%, while 1 microM tautomycin inhibited PP1 activity by 54% and PP2A by only 7%. Okadaic acid, but not tautomycin, induced the activation of both PKBalpha and PKBbeta. Finally, PP2A subunits were found in several subcellular compartments, including plasma membranes (PM) where the phosphorylation of PKB is thought to occur. In summary, our results suggest that PP2A is the principal phosphatase that dephosphorylates PKB in adipocytes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to organic cyclic compound  IDA 2313392 RGD 
cellular response to organic cyclic compound  IDA 2313392Okadaic acidRGD 

Objects Annotated

Genes (Rattus norvegicus)
Akt1  (AKT serine/threonine kinase 1)
Akt2  (AKT serine/threonine kinase 2)


Additional Information