RGD Reference Report - Association of an A-kinase-anchoring protein signaling scaffold with cadherin adhesion molecules in neurons and epithelial cells. - Rat Genome Database

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Association of an A-kinase-anchoring protein signaling scaffold with cadherin adhesion molecules in neurons and epithelial cells.

Authors: Gorski, JA  Gomez, LL  Scott, JD  Dell'Acqua, ML 
Citation: Gorski JA, etal., Mol Biol Cell. 2005 Aug;16(8):3574-90. Epub 2005 Jun 1.
RGD ID: 2313226
Pubmed: PMID:15930126   (View Abstract at PubMed)
PMCID: PMC1182299   (View Article at PubMed Central)
DOI: DOI:10.1091/mbc.E05-02-0134   (Journal Full-text)

A-kinase-anchoring protein (AKAP) 79/150 organizes a scaffold of cAMP-dependent protein kinase (PKA), protein kinase C (PKC), and protein phosphatase 2B/calcineurin that regulates phosphorylation pathways underlying neuronal long-term potentiation and long-term depression (LTD) synaptic plasticity. AKAP79/150 postsynaptic targeting requires three N-terminal basic domains that bind F-actin and acidic phospholipids. Here, we report a novel interaction of these domains with cadherin adhesion molecules that are linked to actin through beta-catenin (beta-cat) at neuronal synapses and epithelial adherens junctions. Mapping the AKAP binding site in cadherins identified overlap with beta-cat binding; however, no competition between AKAP and beta-cat binding to cadherins was detected in vitro. Accordingly, AKAP79/150 exhibited polarized localization with beta-cat and cadherins in epithelial cell lateral membranes, and beta-cat was present in AKAP-cadherin complexes isolated from epithelial cells, cultured neurons, and rat brain synaptic membranes. Inhibition of epithelial cell cadherin adhesion and actin polymerization redistributed intact AKAP-cadherin complexes from lateral membranes to intracellular compartments. In contrast, stimulation of neuronal pathways implicated in LTD that depolymerize postsynaptic F-actin disrupted AKAP-cadherin interactions and resulted in loss of the AKAP, but not cadherins, from synapses. This neuronal regulation of AKAP79/150 targeting to cadherins may be important in functional and structural synaptic modifications underlying plasticity.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dendrite membrane  IDA 2313226 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Akap5  (A-kinase anchoring protein 5)
Cdh1  (cadherin 1)
Cdh2  (cadherin 2)
Ctnnb1  (catenin beta 1)


Additional Information