RGD Reference Report - Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells. - Rat Genome Database

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Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells.

Authors: Moeller, HB  Knepper, MA  Fenton, RA 
Citation: Moeller HB, etal., Kidney Int. 2009 Feb;75(3):295-303. Epub 2008 Oct 8.
RGD ID: 2313123
Pubmed: PMID:18843259   (View Abstract at PubMed)
PMCID: PMC3502047   (View Article at PubMed Central)
DOI: DOI:10.1038/ki.2008.505   (Journal Full-text)

Trafficking of the water channel aquaporin-2 to the apical plasma membrane of the collecting duct is mediated by arginine vasopressin, rendering the cell permeable to water. We recently identified a novel form of aquaporin-2 that is phosphorylated at serine-269 (pS269-AQP2). Using antibodies specific for this form of the water channel, we detected rat and mouse pS269-AQP2 in the connecting tubule and throughout the collecting duct system. Using confocal immunofluorescence microscopy with organelle-specific markers and immunogold electron microscopy, we found that pS269-AQP2 was found only on the apical plasma membrane of principal cells. In vasopressin-deficient Brattleboro rats, pS269-AQP2 was undetectable but dramatically increased in abundance after these rats were treated with [deamino-Cys-1, d-Arg-8]vasopressin (dDAVP). This increase occurred only at the apical plasma membrane, even after long-term dDAVP treatment. Following dDAVP there was a time-dependent redistribution of total aquaporin-2 from predominantly intracellular vesicles to the apical plasma membrane, clathrin-coated vesicles, early endosomal compartments, and lysosomes. However, pS269-AQP2 was found only on the apical plasma membrane at any time. Our results show that S269 phosphorylated aquaporin-2 is exclusively associated with the apical plasma membrane, where it escapes endocytosis to remain at the cell surface.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
clathrin-coated vesicle  IDA 2313123 RGD 
early endosome  IDA 2313123 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aqp2  (aquaporin 2)


Additional Information