RGD Reference Report - Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein. - Rat Genome Database

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Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein.

Authors: Bridges, D  MacDonald, JA  Wadzinski, B  Moorhead, GB 
Citation: Bridges D, etal., Biochem Biophys Res Commun. 2006 Jul 21;346(1):351-7. Epub 2006 Jun 2.
RGD ID: 2312597
Pubmed: PMID:16756943   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2006.05.138   (Journal Full-text)

Protein kinase A (PKA) plays an important role in the regulation of lipid metabolism in adipocytes. The activity of PKA is known to be modulated by its specific location in the cell, a process mediated by A-kinase anchoring proteins (AKAPs). In order to examine the subcellular localization of PKA in this tissue we performed a search for AKAP proteins in adipocytes. We purified a 120 kDa protein which can bind both the regulatory subunit of PKA as well as the catalytic subunit of protein phosphatase 1 (PP1). This protein was found to be enriched in the lipid droplet fraction of primary adipocytes and was identified as D-AKAP1. This protein may play an important role in the regulation of PKA in adipocytes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
regulation of protein kinase A signaling  ICGO:00434952312597 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lipid droplet  IDA 2312597 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
molecular adaptor activity  IDA 2312597 RGD 
protein kinase A regulatory subunit binding  IDA 2312597 RGD 
protein phosphatase binding  IDA 2312597 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Akap1  (A-kinase anchoring protein 1)


Additional Information