RGD Reference Report - [Effects of antimicrobial agents on the Toll like receptors and myeloid differentiation protein-2 in liver tissue of alcohol-induced liver disease: experiment with rats] - Rat Genome Database

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[Effects of antimicrobial agents on the Toll like receptors and myeloid differentiation protein-2 in liver tissue of alcohol-induced liver disease: experiment with rats]

Authors: Lu, ZQ  Li, MF  Liang, H  Qiu, QM  Zhou, TL  Hong, GL  Liu, G 
Citation: Lu ZQ, etal., Zhonghua Yi Xue Za Zhi. 2009 Jan 13;89(2):138-41.
RGD ID: 2312497
Pubmed: PMID:19489280   (View Abstract at PubMed)

OBJECTIVE: To investigate the effects of antimicrobial agents on the Toll like receptors (TLRs) and myeloid differentiation protein (MD)-2 in liver tissue of alcohol-induced liver disease with Vibrio vulnificus (VV) sepsis. METHODS: Eighty SD rats were randomly divided into 2 groups: alcohol gastric lavage group (n = 74) undergoing alcohol gastric perfusion once a day for 10 weeks and normal control group (Group N, n = 6). 66 surviving rats in the gastric perfusion group were randomly divided into 6 equal subgroups: Subgroup A was alcohol-induced liver disease control subgroup. Subgroup AA, alcohol-induced liver disease and antibacterial drug control subgroup, underwent intraperitoneal injection of cefoperazone sodium and levofloxacin (LVFX). The other 9 subgroups underwent subcutaneous injection of VV to establish animal model of VV sepsis, the rats of 4 of which were killed 2, 6, 12, and 24 h later respectively with their livers taken out (Subgroups AV), and the rats of 5 of which underwent intraperitoneal injection of cefoperazone sodium and LVFX 4 h after VV injection twice a day (Subgroups AVA) and were killed 6, 12 , 24 , 36 h, and 1 week later with their livers taken out. The behavioral changed were observed. PCR was used to detect the mRNA expression of TLR2, TLR4, and MD-2. RESULTS: The mRNA expression levels of TLR2, TLR4, and MD-2 in liver 2, 6, 12, and 24 h after in Subgroups AV were all significantly higher than those of Group N (P < 0.05 or P < 0.01) with the maximum level in the AV-12 h subgroup. The mRNA expression levels of TLR2, TLR4, and MD-2 in liver 6 h, 12 h, and 24 h after the VV injection of Subgroup AVA were all significantly lower than those of Group AV (P < 0.05 or P < 0.01). CONCLUSION: The mRNA expression levels of TLR2, TLR4, and MD-2 in liver tissue of alcohol-induced liver disease with VV sepsis, which may be reduced by treatment of cefoperazone sodium and LVFX, are associated with the development of VV sepsis. This treatment is effective on this disease. Dynamic monitoring of the mRNA expression levels of TLR2, TLR4, and MD-2 in liver tissue benefits observation of the VV sepsis progress and treatment.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Sepsis  ISOTlr2 (Rattus norvegicus)2312497; 2312497associated with Liver Diseases and mRNA:increased expression:liverRGD 
Sepsis  ISOTlr4 (Rattus norvegicus)2312497associated with Liver Diseases and mRNA:increased expression:liverRGD 
Sepsis  IEP 2312497; 2312497associated with Liver Diseases and mRNA:increased expression:liverRGD 
Sepsis  ISOTlr4 (Rattus norvegicus)2312497associated with Sepsis and mRNA:increased expression:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Mus musculus)
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Homo sapiens)
TLR2  (toll like receptor 2)
TLR4  (toll like receptor 4)


Additional Information